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Assay Kits & ELISAs

Autophagy Assay - Red Fluorescent

Product Sizes
50 tests
9156-50TESTS
About this Product
SKU:
9156
Application:
Flow Cytometry
CE/IVD:
RUO
Extra Details:
For the detection and monitoring the in vitro development of autophagy in living cells. The autophagy probe fluoresces red when inserted in the lipid membranes of autophagosomes and autolysosomes. Results can be read using a flow cytometer., ImmunoChemistry Technologies
Immunogen:
Autophagy
Physical State:
Cell Culture
Purification:
Jurkat cells were treated with 0.5 uM Rapamycin and 10 uM Chloroquine (red histogram) to induce autophagy or were mock treated (black histogram) for 18 hours. Cells were stained with Autophagy Probe,
Shipping Conditions:
Blue Ice
Storage Conditions:
2-8[o]C, -20[o]C
Type:
Assay Kits: Detection
Figure 1. The process of autophagy is an intracellular degradation process during which cytosolic organelles and materials are enclosed within an isolation membrane to form an autophagosome. The outer membrane of the autophagosome fuses with the lysosome. The sequestered material is subsequently degraded within the autolysosome.
Figure 2. Cells were either untreated (Black) or treated with 0.5 uM Rapamycin (Orange), 10 uM Chloroquine (Blue), or both 0.5 uM Rapamycin and 10 uM Chloroquine (Red) for 18 hours. After staining with Autophagy Probe - Red Fluorescent, cells were washed and analyzed by flow cytometry (561 nm excitation and a 610/20 emission filter). Treatment with rapamycin or chloroquine increased the fluorescence signal compared to the control. Data courtesy of Dr. Kristi Strandberg (ICT 228:37-40).
Autophagy induction in normal MCF-10A breast epithelial cells following a 24 h exposure to the tested compounds (EDAG-1 and EDAG-8) and cisplatin (concentrations of 0.5 and 1 uM). An Autophagy Assay Kit (Cat no 9156) and a flow cytometer were used in the experiment. Image from publication, CC-BY-4.0, PMID: 39063006.
Autophagy induction in normal MCF-10A breast epithelial cells following a 24 h exposure to the tested compounds (EDAG-1 and EDAG-8) and cisplatin (concentrations of 0.5 and 1 uM). An Autophagy Assay Kit (Cat no 9156) and a flow cytometer were used in the experiment. Image from publication, CC-BY-4.0, PMID: 39063006.