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Assay Kits & ELISAs

Nitric Oxide Synthase Assay

Product Sizes
50-100 tests
9155-50-100TESTS
About this Product
SKU:
9155
Application:
Flow Cytometry
Extra Details:
Assess the potency of nitrosative stress inhibitor and activator reagents. Measure the overall intracellular levels of free nitric oxide and NOS using a DAF-2DA dye. Analyze on a flow cytometer, fluorescence plate reader, or fluorescence microscope.
Immunogen:
Nitric Oxide, NOS
Physical State:
Cell Culture
Shipping Conditions:
Blue Ice
Storage Conditions:
2-8[o]C
Type:
Assay Kits: Detection
Figure 1. Jurkat cells were stained with DAF-2DA dye (Kit 9155), washed, and then treated with DMSO control (left histogram) or 1 mM DEA NONOate, a nitric oxide donor (middle histogram). Cells were read on the FL1 channel of a flow cytometer. The median fluorescence intensity (MFI) of DEA NONOate treated cells was 3.5-fold higher than for the control cells. Data also overlaid in a single plot (right, black: Negative; right, red: Positive). Data courtesy of Dr. Kristi Strandberg, ICT, 227:76.
Figure 2. Jurkat suspension cells were stained with DAF-2DA dye, washed, and then treated with DMSO control (Panels A-C) or DEA NONOate (Panels D-F), a nitric oxide donor. Cells treated with DEA NONOate showed an increased level of green fluorescence relative to the untreated cells (Panels A v and D). Microscope images were obtained using a Nikon Eclipse 90i microscope with a Hamamatsu Flash 4.0 camera. Data courtesy of Dr. Kristi Strandberg (ICT 228:57-58).
Figure 3. Jurkat suspension cells were stained with DAF-2DA dye for 1 hour, and then washed. Cells were then treated with DMSO as a negative control or 1 mM DEA NONOate, a nitric oxide donor, for 30 minutes at 37°C. Cells were analyzed using a fluorescence plate reader using an excitation at 488 nm and emission at 515 nm. Data courtesy of Dr. Strandberg, ICT, 227:39-42.