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Assay Kits & ELISAs

Advanced Calcein AM Cell Viability Kit

Product Sizes
25-250 tests
9154-25-250TESTS
About this Product
SKU:
9154
Application:
Flow Cytometry
Extra Details:
This kit combines Calcein AM with 7-AAD to allow for easy and simultaneous labeling of live, membrane compromised, and dead cells within a single sample. Samples can be analyzed using a flow cytometer or fluorescent microscope.
Immunogen:
Intracellular esterases, GC-rich DNA
Physical State:
Cell Culture
Shipping Conditions:
Blue Ice
Storage Conditions:
2-8[o]C
Type:
Assay Kits: Cell Viability
Figure 1. Live cells with green fluorescence (right side of histogram) can be distinguished from dead cells (unstained, left side of histogram). Forward and side scatter graphs are also shown. Jurkat cells were stained with 1 uM Calcein AM and analyzed in a flow cytometer with a FL-1 99% attenuation filter. 93.0% of the live cells stained positive with Calcein AM (A, right), while 0.0% of ethanol-killed cells stained positive (B, right). Data courtesy of Dr. Kristi Strandberg, ICT 226:87-92.
Figure 2. Using 7-AAD, live cells (unstained, left side of each histogram) can easily be distinguished from dead/membrane compromised cells exhibiting red fluorescence (right side of each histogram). Jurkat cells were stained with 7-AAD, and analyzed using a flow cytometer in FL-3. Only 4% of untreated cells (A) are dead compared with 97.6% of ethanol-treated cells (B). Data courtesy of Dr. Kristi Strandberg, ICT 226:30-31.
Figure 3. Untreated Jurkat suspension cells were dually stained with 1 uM Calcein AM for 60 minutes at 37°C to detect live cells. Cells were then stained with 4 uM 7-AAD for 10 minutes on ice to detect membrane-compromised or dead cells. Panel A reveals green fluorescence-stained live cells. A few dead cells stained red due to 7-AAD uptake. Panel B shows a corresponding DIC image, which reveals cell morphology. Data courtesy of Dr. Kristi Strandberg (ICT 226:95).
Figure 4. Jurkat suspension cells were dually stained with 1 uM Calcein AM to detect live cells, and then stained with 4 uM 7-AAD to detect membrane-compromised or dead cells. Untreated live cells stained green (A). Occasional dead cells stained red due to 7-AAD uptake. Ethanol-killed cells all stained red due to 7-AAD uptake (B). A 50/50 mixture of the untreated and ethanol-killed cells shows both green and red cells (C). Data courtesy of Dr. Kristi Strandberg, ICT 226:95.
Figure 5. Jurkat cells were treated with DMSO (non-induced) or 1 uM staurosporine to induce apoptosis, then dually stained with 1 uM Calcein AM to detect live cells and 4 uM 7-AAD to detect membrane-compromised or dead cells. All of the non-induced cells fluoresced green only (A). Most of the induced cells were green (live), many cells were both green and red (compromised membranes, likely early apoptotic), and some were red (dead). Data courtesy of Dr. Kristi Strandberg, ICT 226:95.