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Assay Kits & ELISAs

Green Fluorescent Cathepsin B Assay

Product Sizes
25 tests
9151-25TESTS
About this Product
SKU:
9151
Application:
Flow Cytometry
CE/IVD:
RUO
Extra Details:
The quenched substrate Rhodamine 110-(RR)2, fluoresces green upon cleavage by active cathepsins, allowing the monitoring of intracellular cathepsin activity in vitro using live whole cells. Analyze using flow cytometry or fluorescence microscopy., ImmunoChemistry Technologies
Immunogen:
Cathepsin B
Physical State:
Cell Culture
Purification:
Cathepsin activity in THP-1 monocytes. Intracellular cathepsin activity was detected in THP-1 monocytes that were either untreated to show baseline cathepsin activity (green histogram) or were treated
Shipping Conditions:
Blue Ice
Storage Conditions:
2-8[o]C, -20[o]C
Type:
Assay Kits: Detection
Figure 1. R110-(RR)2 was reconstituted in DMSO, diluted in an aqueous buffer, and then spiked with cathepsin B enzyme and incubated at 37°C for 1 hour. After incubation, fluorescence was analyzed on a Molecular Devices M5e plate reader. The excitation spectrum (black) was generated using an emission of 590 nm. The emission spectrum (green) was generated using an excitation of 460 nm.