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Assay Kits & ELISAs

Intracellular Total ROS Activity Assay

Product Sizes
100 tests
9144-100TESTS
About this Product
SKU:
9144
Application:
Flow Cytometry
CE/IVD:
RUO
Extra Details:
The Intracellular Total ROS Activity Assay enables the screening of oxidative stress inhibitor and activator reagents for potency. The assay assesses the overall level of intracellular ROS activity. Analyze using a flow cytometer., ImmunoChemistry Technologies
Immunogen:
ROS
Physical State:
Cell Culture
Purification:
Jurkat cells were stained with ICT's Intracellular Total ROS Activity Assay (kit #9144) for 1 hour, and then treated with a negative control (left histogram) or 100 uM tert-Butyl hydroperoxide, a reac
Shipping Conditions:
Blue Ice
Storage Conditions:
2-8[o]C, -20[o]C
Type:
Assay Kits: Detection
Figure 1. Jurkat cells were stained with the Intracellular Total ROS Green dye (cat. 9144), then treated with a negative control (left) or 100 uM tert-Butyl hydroperoxide (ROS-inducing agent) (middle). The median fluorescence intensity (MFI) of stained negative control cells was 11,875 in FL1-A (Negative) and 419,067 for ROS-induced cells (Positive). The data are also shown in overlay (black: Negative, red: Positive). Data courtesy of Dr. Kristi Strandberg, ICT, 224:76.