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Assay Kits & ELISAs

Far-Red Fluorescent FLICA[R] 660 Caspase-3/7 (DEVD) Assay Kit

Product Sizes
25-50 tests
9125-25-50TESTS
About this Product
SKU:
9125
Application:
Flow Cytometry
CE/IVD:
RUO
Extra Details:
This in vitro assay employs the far-red fluorescent inhibitor probe 660-DEVD-FMK to label active caspase 3 and caspase 7 enzymes in living cells. Analyze the fluorescent signal using fluorescence microscopy or flow cytometry., ImmunoChemistry Technologies
Immunogen:
Caspase 3 and 7
Physical State:
Cell Culture
Purification:
To demonstrate the fluorescence properties and apoptosis detection capability of the new far-red fluorescent FLICA 660 Caspase-3 Assay, Jurkat cells were were treated with 1 uM staurosporine for 4 hou
Shipping Conditions:
Blue Ice
Storage Conditions:
2-8[o]C
Type:
Assay Kits: Cell Viability
Figure 1. FLICA® 660 was reconstituted in DMSO, diluted in diH2O, and analyzed in a fluorescence plate reader. The excitation spectrum was generated using an emission of 760 nm. The emission spectrum was generated using an excitation of 600 nm.
Figure 2. Jurkat cells were treated with a negative control (left) or staurosporine, an apoptosis-inducing agent (right), for 4 hours, then stained with FLICA® 660-DEVD-FMK (cat. 9125) for 1 hour. Cells were washed and analyzed by flow cytometer. The negative control induced caspase-3/7 activity in 4.4% of cells (left); treatment with staurosporine induced caspase-3/7 activity in 70.9% of cells (right). This is a ratio of 16:1. Data courtesy of Mrs. Tracy Murphy, ICT, 13F38, 022013.