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Assay Kits & ELISAs

TMRM - Orange Fluorescent Mitochondrial Membrane Depolarization Assay

Product Sizes
500 tests
9105-500TESTS
About this Product
SKU:
9105
Application:
Flow Cytometry
Extra Details:
TMRM accumulates and fluoresces orange in polarized mitochondria. Fluorescence drops dramatically on depolarization in apoptotic or metabolically stressed cells. Analyze using a flow cytometer, fluorescence plate reader, or fluorescence microscopy.
Immunogen:
Mitochondrial depolarization
Physical State:
Cell Culture
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C
Type:
Assay Kits: Cell Viability
Figure 1. Jurkat cells were exposed to DMSO as the negative control (dark orange bars) or 50 uM CCCP depolarizing agent (light orange bars) and then incubated with TMRE or TMRM. Aliquots (100 uL) were analyzed in triplicate in a black 96-well plate using a fluorescence plate reader (550 nm excitation and 580 nm emission using a 570 nm cut-off filter). Healthy control cells had a high level of orange fluorescence compared to the CCCP-treated cells. (Ms. Tracy Hanson, ICT).
Figure 2. Jurkat cells were treated with DMSO (control) (A) or staurosporine (B), and then dually stained with TMRM and the green FLICA® poly caspase probe FAM-VAD-FMK (cat. 92) and analyzed by flow cytometer. In the staurosporine-treated cells (B), there is loss of orange fluorescence (FL-2) and a concurrent increase in cell-bound green fluorescence (FL-1) due to caspase activation. Treatment with staurosporine induced apoptosis in about 90% of cells (Ms. Tracy Hanson, ICT).