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Antibodies

Propidium Iodide Stain

Product Sizes
1 ml
638-1ML
About this Product
SKU:
638
Application:
Flow Cytometry
Concentration:
250 ug/ml
Extra Details:
Propidium Iodide is an intercalating fluorescent agent that binds between the bases of DNA. Propidium Iodide is membrane impermeant, which prevents DNA binding in viable cells, allowing identification of dead cells in a population.
Immunogen:
Necrotic and apoptotic cells
Shipping Conditions:
Blue Ice
Storage Conditions:
2-8[o]C
Type:
Antibody: Stains & Enhancers
Figure 1. Propidium iodide excitation and emission spectra.
Figure 2. HL-60 cells were treated with a drug, then stained with FLICA® FAM-VAD-FMK poly-caspase inhibitor reagent (green) (Cat. 92) and propidium iodide (red) (PI). Scanning laser cytometer analysis. Four populations of cells were detected: (A) Unstained live cells, (B) red necrotic cells (PI), (C) green and red late apoptotic cells (FAM-FLICA® plus PI), and (D) green early apoptotic cells (FAM-FLICA®). Data courtesy of Dr. Z. Darzynkiewicz, Brander Cancer Center, NY.
Figure 3. Cell death in primary rat hippocampal neurons. (D) is a composite of FLICA® FAM-DEVD-FMK (B), PI (C), and Hoechst (A). DNA is labeled in 4 cells (A), 3 cells are caspase-positive (B), 2 are membrane-compromised (C). One cell is in early apoptosis (green but not red). Two FLICA®-positive cells are also PI-positive (red, C) (becoming membrane compromised) and are in the late stages of apoptosis rather than necrosis. Data courtesy of Dr. Z. Kahraman Akozer, University of Maryland.