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Antibodies

7-AAD Red Fluorescent Live/Dead Stain

Product Sizes
0.26 mg
6163-0.26MG
1 each
6163-1EACH
About this Product
SKU:
6163
Application:
Flow Cytometry
CE/IVD:
RUO
Extra Details:
7-Aminoactinomycin D (7-AAD) is a red fluorescent vital dye that intercalates in double-stranded DNA with a high affinity for GC-rich regions. 7-AAD does not pass through intact cell membranes. It may also be used to label all cells after fixation., ImmunoChemistry Technologies
Molecular Weight:
1270
Purification:
Jurkat cells were grown to 5 x 105 cells/mL and split into two populations. One population (left) was left untreated (live) while the other population (right) was treated with 90% ethanol for 60 secon
Shipping Conditions:
Blue Ice
Storage Conditions:
2-8[o]C
Type:
Antibody: Stains & Enhancers
Figure 1. Jurkat cells were untreated (live) (A) or treated with 90% ethanol (dead) (B). Cells were pelleted by centrifugation (200 x g for 5 minutes) and resuspended in PBS. Cells were then stained with 7-AAD for 10 minutes on ice, and analyzed using an Accuri C6 flow cytometer in FL-3. Only 4% of untreated cells (A) are dead compared with 97.6% of the treated cells (B) (ICT 226:30-31).
Figure 2. Jurkat cells were exposed to staurosporine to induce apoptosis, then dually stained with the green fluorescent FAM-FLICA poly caspase probe to detect apoptosis via caspase activity, and the red dye 7-AAD to detect necrosis. Early apoptotic cells fluoresce green with FAM-FLICA. Dually stained green and red fluorescing cells are in mid to late stage apoptosis (active caspases and compromised cell membranes). Necrotic cells fluoresce red. (ICT 196:70).
Figure 3. Jurkat cells were treated with DMSO (A) or staurosporine (B), then stained with poly caspase FAM-FLICA apoptosis reagent (Cat. 637) and 7-AAD. FAM-FLICA was analyzed on FL-1, and 7-AAD on FL-3. Four cell populations are identified - see key for details. FAM-FLICA detects cells in early apoptosis (lower right quadrant), which 7-AAD cannot detect alone. Only 10.8% of control cells were apoptotic (LR: 4.5% +UR: 6.3%) compared with 97.1% of induced cells (B; LR: 46.4% + UR: 50.7%) (ICT).