Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

CFSE Green Fluorescent Stain - for cell labelling and proliferation studies

Product Sizes
50 ug
6162-50UG
About this Product
SKU:
6162
Application:
Flow Cytometry
Extra Details:
CFSE is a fluorogenic reagent which is frequently used in cell labeling and cell proliferation procedures. Non-fluorescent and moderately hydrophobic in its di-esterified form, the dye easily penetrates lipid bi-layers of living cells and is quickly converted to the amine-reactive, green fluorescent form (CFSE) upon cleavage of the acetate groups by intracellular esterases. This highly fluorescent form is membrane-impermeant and is retained on and within the cells, while any excess probe is easily washed away in subsequent wash steps. Because CFSE forms a strong bond inside the cell, it is retained within the cell indefinitely and is inherited by daughter cells. It will not be incorporated into adjacent cells.
Molecular Weight:
557.47
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C
Type:
Antibody: Stains & Enhancers
Figure 1. Staining of Healthy Jurkat Cells Healthy Jurkat cells were stained with CFSE or left unstained and then analyzed by flow cytometry using an Accuri C6 flow cytometer equipped with a FL1 99% attenuation filter. Unstained Jurkat cells (A), stained Jurkat cells (B), and the corresponding overlay (C) are shown.
Figure 2. Identification of CFSE-stained Cells. K562 target cells were stained green with CFSE and then subjected to effector cells. Green stained target cells are easily identifiable when analyzed by FL1 vs. SSC on a flow cytometer. Target cells stained green with CFSE move to the right along the X-axis (FL1) of the plot (right) compared with unstained effector cells. This plot becomes particularly important when gating on target cells that are the same size as effector cells.
Figure 3. K562 target cells were stained with CFSE to distinguish them from effector cells in FL1 (Fig 2). The target cells were stained with 7-AAD (cat 6163) to identify membrane-compromised necrotic target cells (red in FL3), and with SR-FLICA® (cat 917) to identify caspase-positive apoptotic target cells (orangy-red in FL2). When used together with Total Cytotoxicity Assay kit (cat 972), these reagents identified four populations of cells: live; early apoptotic; late apoptotic; and necrotic.