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Antibodies

anti-TIM3 antibody

Product Sizes
100 ug
£700.00
ARG63927-100UG
Academic Pricing
Please contact us for academic pricing.
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About this Product
SKU:
ARG63927
Additional Names:
KIM-3; TIM3; TIMD-3; Tim-3; TIM-3; CD366; T-cell immunoglobulin and mucin domain-containing protein 3; TIMD3; T-cell membrane protein 3; HAVcr-2; T-cell immunoglobulin mucin receptor 3; Hepatitis A virus cellular receptor 2
Application:
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Western Blot
Buffer:
Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
0.5 mg/ml
Extra Details:
The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance. [provided by RefSeq, Sep 2011]
Host:
Goat
Isotype:
IgG
Purification:
Ammonium Sulfate Precipitated; Affinity Purified
Reactivities:
Canine
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C
Supplier:
Arigo Biolaboratories
Type:
Antibody: Polyclonal Antibody
Western blot: 1). Mock transfection; 2) HAVCR2 (RC209440) expressing plasmid transfected HEK293 cell lysate standed with ARG63927 anti-TIM3 antibody.
Flow Cytometry: Paraformaldehyde-fixed HepG2 cells permeabilized with 0.5% Triton. Cells were stained with ARG63927 anti-TIM3 antibody (blue line) at 10 ug/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
Immunofluorescence: Paraformaldehyde fixed HepG2 cells permeabilized with 0.15% Triton. Cells were stained with ARG63927 anti-TIM3 antibody (green) at 10 ug/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 ug/ml dilution.
Western blot: 35 ug of Jurkat (A), MOLT-4 (B) and A431 (C, negative control) cell lysates (in RIPA buffer) stained with ARG63927 anti-TIM3 antibody at 2 ug/ml dilution and incubated at RT for 1 hour.
Western blot: 35 ug of Human tonsil lysate (in RIPA buffer) stained with ARG63927 anti-TIM3 antibody at 1 ug/ml dilution and incubated at RT for 1 hour.