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Antibodies

anti-Uroplakin III, AU1, mouse monoclonal antibody

Product Sizes
20 ug
£122.00
690108-20UG
100 ug
£275.00
690108-100UG
About this Product
SKU:
690108
Additional Names:
Uroplakin-3a, UP3a, Uroplakin III, UPIII, UPK3A, UPK3
Application:
Immunohistochemistry, Western Blot
CE/IVD:
RUO
translate.label.attr.clone:
AU1
Clonality:
Monoclonal
Concentration:
100 ug/ml
Extra Details:
Mab AU1 reacts specifically with uroplakin IIIA present in the superficial cell layer of the urothelium. The binding region has been localized on the extracellular part of the antigen. Together with the uroplakins UP Ia, UP Ib and UP II, uroplakin III contributes in constituting the asymmetrical unit membrane of the plaques of urothelial superficial (umbrella) cells. Uroplakin is a membrane glycoprotein (47 kDa) and has been shown to be a specific marker of terminal urothelial differentia-tion (Wu et al. 1993 & 1194). Antibody AU1 strongly stains the urothelial surface membrane in paraffin sections of human renal pelvis, ureter, bladder, and urethra. About 60% of human transitional cell carcinomas (including metastases) maintain focal (sometimes very limited) expression of uroplakin III. Until now, no uroplakin staining was found in any non-urothelial carcinoma (Moll et al. 1995). Uroplakin III may thus serve as a specific urothelial differentiation marker in cases of metastatic carcinomas with unclear primary tumor. Moll, R., Wu, X.-R., Lin, J.-H. & Sunt, T.-T. Uroplakins, Specific Membrane Proteins of Urothelial Umbrella Cells, as Histological Markers of Metastatic Transitional Cell Carcinomas. Am. J. Pathol. 147, 1383-1397 (1995). Wu, X.-R. et al. Mammalian Uroplakins: A group of highly conserved urothelial differentiation-related membrane proteins. J. Biol. Chem. 269, 13716-13724 (1994). Wu, X. R. & Sun, T. T. Molecular cloning of a 47 kDa tissue-specific and differentiation-dependent urothelial cell surface glycoprotein. J. Cell Sci. 106, 31-43 (1993).
Formulation:
0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4
Host:
Mouse
Immunogen:
Asymmetric unit membrane (AUM) preparation from bovine urinary bladder
Isotype:
IgG1
Purification:
Affinity Purified
Reactivities:
Bovine, Human, Porcine, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
Please refer to datasheet
Supplier:
Progen
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:690108
Western blot analysis of human bladder whole tissue lysate with anti-Uroplakin III antibody. Western blot analysis was performed on 3 ug and 2 ug bladder lysate. The PVDF membrane was blocked with 5% dry milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-Uroplakin III mouse monoclonal
AU1 (Cat. No. 690108) was diluted in blocking buffer (antibody concentration 0.005 ug/ml) and incubated at RT for 1 h. The secondary antibody goat anti-mouse HRP was also diluted in blocking buffer (antibody concentration 0.2 ug/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using PierceTM ECL Western Blotting Substrate.
IHC of rat bladder (courtesy of J.HeB Beta