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Antibodies

anti-Keratin K7, Ks7.18, mouse monoclonal antibody

Product Sizes
50 ug
£263.00
61025-50UG
About this Product
SKU:
61025
Additional Names:
Keratin, type II cytoskeletal 7, Cytokeratin-7, CK-7, Keratin-7, K7, Sarcolectin, Type-II keratin Kb7, KRT7, SCL
Application:
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
CE/IVD:
RUO
translate.label.attr.clone:
Ks7.18
Clonality:
Monoclonal
Concentration:
50 ug/ml
Extra Details:
Ks7.18 represents an excellent marker for the discrimination of specific subtypes of adenocarcinoma: e.g. adenocarcinoma of pancreas, bile duct carcinoma and transitional carcinoma of bladder are stained, whereas hepatocellular and prostate carcinomas are negative. Detects specific subtypes of adenocarcinomas: adenocarcimona of pancreas, gallbladder, lung, cervix; cholangio carcinoma of liver; ductal and lobular carcinoma of breast; carcinomas of ovary; transitional cell carcinoma of bladder; mesothelioma; negative with most cases of hepatocellular carcinoma. In colorectal carcinoma early stages are reported to be negative but advanced stages of tumor development are positive for keratin K7 expression. Occasionally, staining of blood vessel walls, particularly of endothelial cells may be observed. Reacts with Mr 54,000 polypeptide (keratin K7; formerly also designated cytokeratin 7) of human glandular epithelia. Reactivity on cultured cell lines: HeLa, RT 112, T24, BT-20, CAMA-1, Detroit 562, (MCF-7 and HT-29 are negative).
Formulation:
Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Host:
Mouse
Immunogen:
Cytoskeletal proteins from cultured HeLa cells
Isotype:
IgG1
Physical State:
Lyophilized
Purification:
Affinity Purified
Reactivities:
Bovine, Canine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
Shipping Conditions:
Blue Ice
Storage Conditions:
2-8[o]C reconstituted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Progen
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:61025
Western blot analysis of RT-4 lysate with anti-Keratin K7 antibody. Western blot analysis was performed on 20 ug RT-4 lysate. Cells were lysed with RIPA buffer. The PVDF membrane was blocked with 5% dry milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-Keratin K7 mouse monoclonal
Ks7.18 (Cat. No. 690025) was diluted in blocking buffer (antibody concentration 50 ng/ml) and incubated for 1 h at RT. The secondary antibody anti-mouse IgG goat polyclonal
HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 ug/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using PierceTM ECL Western Blotting Substrate.
Human thyroid carcinoma (courtesy of J.HeB Beta
University Hospital Heidelberg)