HighYield T7 Biotin11 RNA Labeling Kit (UTP-based), Preparation of randomly Biotin-modified RNA probes by in vitro transcription with Biotin-11-UTP
Product Sizes
30 reactions
RNT-101-BIOX-30REACTIONS
About this Product
- SKU:
- RNT-101-BIOX
- Conjugate:
- Biotin
- Extra Details:
- HighYield T7 Biotin11 RNA Labeling Kit is designed to produce randomly Biotin-modified RNA probes with a 11-atom linker via in vitro transcription. Such probes are ideally suited for in situ hybridization and Northern Blot experiments. Biotin-11-UTP is efficiently incorporated into RNA as substitute for its natural counterpart UTP using an optimized reaction buffer and T7 RNA Labeling Polymerase Mix. 35 % Biotin-11-UTP substitution typically results in an optimal balance between reaction and labeling efficiency. Individual optimization of Biotin-11-UTP/UTP ratio however, can easily be achieved with the single nucleotide format. The resulting Biotin-modified RNA probe with a 11-atom linker can subsequently be detected by HRP- or AP-modified streptavidin. The kit contains sufficient reagents for 30 labeling reactions of 20 ul each (35 % Biotin-11-UTP substitution, 1 mM ATP, GTP, CTP, 0.65 mM UTP, 0.35 mM Biotin-11-UTP).
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- -20[o]C Aliquot. Avoid freeze/thaw cycles.
- Supplier:
- Jena Bioscience
- Type:
- Assay Kits: Labelling
