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Antibodies

Anti-Slingshot-1L (C-terminal region) Antibody

Product Sizes
100 ul
SP1711-100UL
About this Product
SKU:
SP1711
Additional Names:
SSH1L
Application:
Immunocytochemistry, Immunohistochemistry, Western Blot
Buffer:
PBS
CE/IVD:
RUO
Clonality:
Polyclonal
Extra Details:
Our Slingshot-1L (C-terminal region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Slingshot-1L (C-terminal region) and is antigen affinity purified. It is great for use in WB, ICC, IHC.
Format:
Antigen Affinity Purified
Gene Details:
SSH1
Host:
Rabbit
Immunogen:
A synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal region of human SSH1L. This sequence has 100% homology with similar regions of mouse and bovine SSH1L, and has low homology to SSH2L and SSH3L.
Isotype:
IgG
Molecular Weight:
150
Physical State:
Liquid
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
The antibody detects a 150 kDa* protein corresponding to the molecular mass of SSH1L on SDS-PAGE immunoblots of PC12 cells.
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Type:
Antibody: Polyclonal Antibody
Formalin fixed, citric acid treated parafin sections of mouse cerebral cortex. Sections were probed with anti-Slingshot-1L (SP1711) then anti-Rabbit:HRP before detection using DAB. (Image provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).
DU-145 cells were transfected with control (Ctrl siRNAs) or AKAP2 specific siRNAs (siRNA1 and siRNA2). 72 h after transfection, cell lysates were loaded on SDS-PAGE gels and analyzed by Western blot. Phospho-SSH1 was detected using antibodies recognizing phospho-serines 937 and 978 (cat. SP3901). The amounts of SSH1 (cat. SP1711) and AKAP2 were detected using specific antibodies, as indicated. Image from publication CC-BY-4.0. PMID: 37827203
Western blot of human recombinant SSH1L untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) and rat PC12 cells unstimulated (lanes 5 & 7) or stimulated with calyculin A (lanes 6 & 8). The blots were probed with anti-SSH1L (C-term.) (lanes 1, 2, 5, & 6) or anti-SSH1L (Ser-978) (lanes 3, 4, 7, & 8).