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Antibodies

Anti-p62 (Thr269, Ser272) Antibody

Product Sizes
25 ul
p196-269-25UL
100 ul
p196-269-100UL
About this Product
SKU:
p196-269
Additional Names:
A170 antibody, DMRV antibody, EBI 3 associated protein of 60 kDa antibody, EBI 3 associated protein p60 antibody, EBI3 associated protein of 60 kDa antibody, EBI3 associated protein p60 antibody, EBI3-associated protein of 60 kDa antibody, EBIAP antibody, FTDALS3 antibody, MGC127197 antibody, ORCA antibody, OSF-6 antibody, Osi antibody, OSIL antibody, Oxidative stress induced like antibody, p60 antibody, p62 antibody, p62B antibody, Paget disease of bone 3 antibody, PDB 3 antibody, PDB3 antibody, Phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa antibody, Phosphotyrosine independent ligand for the Lck SH2 domain p62 antibody, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa antibody, PKC-zeta-interacting protein antibody, Protein kinase C-zeta-interacting protein antibody, Sequestosome 1 antibody, Sequestosome-1 antibody, SQSTM 1 antibody, SQSTM_HUMAN antibody, Sqstm1 antibody, STAP antibody, STONE14 antibody, Ubiquitin binding protein p62 antibody, Ubiquitin-binding protein p62 antibody, ZIP 3 antibody, ZIP antibody, ZIP3 antibody
Application:
Western Blot
Buffer:
10 mm hepes
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
Thr269, Ser272 - Phosphorylated
Extra Details:
Our Anti-p62 (Thr269, Ser272) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human p62 (Thr269, Ser272) and is antigen affinity purified from pooled serum. It is great for use in WB.
Format:
Antigen Affinity Purified from Pooled Serum
Gene Details:
SQSTM1
Host:
Rabbit
Immunogen:
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr269/Ser272 of human p62, conjugated to keyhole limpet hemocyanin (KLH).
Isotype:
IgG
Molecular Weight:
62
Physical State:
Liquid
Purification:
Affinity Purified
Reactivities:
Human, Non-human Primate
Shipping Conditions:
Blue Ice
Specificity:
Specific for endogenous levels of the ~62 kDa p62 protein phosphorylated at Thr269 and Ser272. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Type:
Antibody: Polyclonal Antibody
Western blot of Jurkat cell lysate showing specific immunolabeling of the ~62 kDa p62 phosphorylated at Thr269/Ser272 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (not shown).
AD293, A375 and MDA-MB-231 cells were treated with DMSO (control), or MG132 (2 uM), and Doramapimod (50 uM), alone or in combination for 14 h. The whole-cell lysates were subjected to western blot analysis with indicated antibodies. Image from publication CC-BY-4.0. PMID: 35840557
AD293 cells and A375 cells were treated with MG132 (2 uM), and Doramapimod (50 uM), alone or in combination for 14 h. The whole-cell lysates were subjected to western blot analysis with total-SQSTM1, SQSTM1 S403, SQSTM1 T269/S272 (cat. p196-269, 1:1000), and GAPDH. Image from publication CC-BY-4.0. PMID: 37872526.