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Antibodies

Anti-Myosin Light Chain (Ser-1), Phosphospecific Antibody

Product Sizes
100 ul
MP3461-100UL
About this Product
SKU:
MP3461
Additional Names:
MLC20, RLC-C; Mylc2c; Myl9, MLC2, MRLC1, MYRL2; MLCB; MRCL3; MRLC3; MYL2B; MYL12A, myosin
Application:
Immunocytochemistry, Western Blot
Buffer:
PBS
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
Ser-1 - Phosphorylated
Extra Details:
Our Myosin Light Chain (Ser-1) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects bovine, human, mouse, and rat Myosin Light Chain (Ser-1) and is antigen affinity purified. It is great for use in WB, ICC.
Format:
Antigen Affinity Purified
Gene Details:
MYL12A
Host:
Rabbit
Immunogen:
Phospho-MLC (Ser-1) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Ser-1 in bovine myosin regulatory light chain 9 (Myl9). This peptide sequence is highly conserved in human, rat, and mouse Myl9, and in other smooth muscle and non-muscle MLCs.
Isotype:
IgG
Molecular Weight:
20
Physical State:
Liquid
Reactivities:
Bovine, Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
The antibody detects a 20 kDa* band corresponding to the molecular weight of myosin light chain in human A431 treated with EGF or calyculin A. In addition, the antibody detects the 20 kDa myosin light chain in mouse C2C12, rat PC12, and rabbit fibroblasts treated with calyculin A.
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Type:
Antibody: Polyclonal Antibody
Western blot analysis of C2C12 cells untreated (lanes 1, 3, 5, & 7) or treated with Lambda phosphatase (lanes 2, 4, 6, & 8). The blots were probed with monoclonal anti-MLC20 (clone MY-21) (lanes 1 & 2), polyclonal anti-MLC (Ser-19) phosho-specific (lanes 3 & 4), anti-MLC (Ser-1) phosho-specific (lanes 5 & 6), or anti-MLC (a.a. 11-22) (lanes 7 & 8) .
Immunocytochemical labeling of phosphorylated MLC in paraformaldehyde fixed A7r5 cells. The cells were dual-labeled with anti-MLC (MM3441; middle) and anti-MLC (MP4201; top left), anti-MLC (Ser-19) (MP4221; middle left) and anti-MLC (Ser-1) (MP3461; bottom left). Goat anti-Mouse DyLight® 488 and Goat anti-Rabbit DyLight® 594 were used for detection of primary antibodies. The overlay of staining patterns are shown to the right.