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Antibodies

Anti-EGFR (N-terminal region) Antibody

Product Sizes
100 ul
EP1871-100UL
About this Product
SKU:
EP1871
Additional Names:
EGF, Epidermal, ErbB
Application:
ELISA, Immunocytochemistry, Western Blot
Buffer:
PBS
CE/IVD:
RUO
Clonality:
Polyclonal
Extra Details:
Our EGFR (N-terminal region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat EGFR (N-terminal region) and is antigen affinity purified. It is great for use in WB, ICC., ECM
Format:
Antigen Affinity Purified
Formulation:
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Gene Details:
EGFR
Host:
Rabbit
Immunogen:
EGFR synthetic peptide (coupled to KLH) corresponding to amino acid residues from the N-terminal region of human EGFR (ErbB-1). This human EGFR sequence has high homology to rat and mouse EGFR, and low homology to other ErbB family members.
Isotype:
IgG
Molecular Weight:
180
Physical State:
Liquid
Purification:
Affinity purified rabbit serum
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
The antibody detects a 180 kDa* protein on SDS-PAGE immunoblots of human A431 cells.
Storage Conditions:
-20[o]C 50% glycerol. Aliquot. Avoid freeze/thaw cycles.
Type:
Antibody: Polyclonal Antibody
Immunocytochemical labeling in A431 cells untreated or treated with Calyculin A (50 nM) for 15 min. Cells were labeled with anti-EGFR (a.a. 961-972) or anti-EGFR (Ser-1142) antibodies. The specificity of labeling was demonstrated for each antibody by blocking the signals with their respective blocking peptides, EGFR (a.a. 961-972) (EX1875) and phospho-EGFR (Ser-1142) (EX1935).
Western blot image of human A431 cells treated with Calyculin A (100 nM) for 30 min. Blot lanes were untreated (lanes 1, 3, & 5) or treated with lambda phosphatase (lanes 2, 4, & 6) then were probed with anti-EGFR (a.a. 961-972) (lanes 1 & 2), anti-EGFR (Ser-967) (lanes 3 & 4), and anti-EGFR (Ser-1142) (lanes 5 & 6).