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Antibodies

Anti-CRMP2 (Ser-522), Phosphospecific Antibody

Product Sizes
100 ul
CP2191-100UL
About this Product
SKU:
CP2191
Additional Names:
DRP-2, Toad-64, CRMP-62
Application:
Immunocytochemistry, Western Blot
Buffer:
PBS
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
Ser-522 - Phosphorylated
Extra Details:
Our CRMP2 (Ser-522) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat CRMP2 (Ser-522) and is antigen affinity purified. It is great for use in WB, ICC.
Format:
Antigen Affinity Purified
Gene Details:
DPYSL2
Host:
Rabbit
Immunogen:
Phospho-CRMP2 (Ser-522) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding Ser-522 in human CRMP2. This sequence is conserved in rat and mouse CRMP2, and has homology to the conserved site in CRMP1 and CRMP4, but is not conserved in CRMP3 and CRMP5.
Isotype:
IgG
Molecular Weight:
70
Physical State:
Liquid
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
The antibody detects a 70 kDa* protein corresponding to the molecular mass of CRMP2 on SDS-PAGE immunoblots of human mouse brain, and this band is removed after lambda phosphatase treatment. In addition, this reactivity can be specifically blocked using phospho-CRMP2 (Ser-522) peptide (CX2195).
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Type:
Antibody: Polyclonal Antibody
Immunocytochemical labeling of CRMP2 phosphorylation in aldehyde-fixed and NP-40-permeabilized NGF-differentiated PC12 cells. The cells were labeled with rabbit polyclonal anti-CRMP2 (Ser-522) (CP2191) antibody in the absence (Left) or presence (Right) of blocking peptide (CX2195). The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.
Western blot image of mouse brain untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4). The blot was probed with anti-CRMP2 (C-terminal Region) (lanes 1 & 2) or anti-CRMP2 (Ser-522) (lanes 3 & 4).