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Antibodies

Anti-Actin (Tyr-53), Phosphospecific Antibody

Product Sizes
100 ul
AP1671-100UL
About this Product
SKU:
AP1671
Application:
Immunocytochemistry, Western Blot
Buffer:
PBS
CE/IVD:
RUO
Clonality:
Polyclonal
Conjugate:
Tyr-53 - Phosphorylated
Extra Details:
Our Actin (Tyr-53) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, and rat Actin (Tyr-53) and is antigen affinity purified. It is great for use in WB, ICC.
Format:
Antigen Affinity Purified
Gene Details:
ACTB
Host:
Rabbit
Immunogen:
Phospho-Actin (Tyr-53) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 53 of human B Beta actin. This sequence is identical to similar regions in all four A Alpha actins, as well as in G Gamma actin, and is well conserved in actins from most eukaryotic species.
Isotype:
IgG
Molecular Weight:
42
Physical State:
Liquid
Reactivities:
Avian, Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
This antibody detects a 42 kDa* protein corresponding to the molecular mass of Actin on SDS-PAGE immunoblots of pervanadate treated human C2C12 and SYF cSrc transformed cells, but not in control cells. In addition, this antibody either detects actin cleavage products at 36 and 20 kDa, or cross-reacts with unidentified proteins at these molecular weights in C2C12 cells.
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Type:
Antibody: Polyclonal Antibody
Immunocytochemical labeling using anti-Actin (N-terminal) and anti-Actin (Tyr-53) polyclonal antibodies in C2C12 cells control (left) or treated with pervanadate (1 mM) for 30 min (middle). The cells were fixed in paraformaldehyde and permeabilized in acetone. Both antibodies were used in the presence of blocking peptide: Actin (N-terminal) peptide (AX1655) or phospho-Actin (Tyr-53) peptide (AX1675), respectively (right).
Western blot analysis of mouse C2C12 cells untreated (lanes 1 & 3), or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). The blot was probed with anti-Actin (N-terminal) antibody (lanes 1 & 2) or anti-Actin (Tyr-53) antibody (lanes 3 & 4).