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Assay Kits & ELISAs

Bioluminescent Acetylcholinesterase Assay Kit

Product Sizes
100 tests
CLACHE100-2-100TESTS
About this Product
SKU:
CLACHE100-2
Additional Names:
Cell Viability Assay
Application:
ELISA
CE/IVD:
RUO
Extra Details:
A rapid and extremely sensitive assay for acetylcholinesterase using the natural substrate acetylcholine. A series of coupled reactions generate a bioluminescent signal. Ideal for identifying and characterizing acetylcholinesterase inhibitors., ImmunoChemistry Technologies
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C
Type:
Assay Kits: Detection
Figure 1. Assay principle showing the effect of inhibitors
Figure 2. Assay principle schematic
Figure 3. Tacrine (Sigma, A3773, a mixed-mode inhibitor of AChE) was serially diluted in DI water. Next 10 mL of the diluted Tacrine (x axis labeling represents mM final concentration of Tacrine) was added to a white opaque 96 well microplate along with 50 mL of component A (AChE enzyme). The samples were incubated for 5 minutes after which 50 mL of component B was added to all the wells. Data were collected using a luminometer. Data represent t = 2 minutes after the addition of component B.
Figure 4. Malathion, a common pesticide, was diluted in DMSO and then serially diluted in Di water. 10 mL of the diluted Malathion (x axis represents mM final concentration of Malathion) was added to a white opaque 96 well microplate followed by 50 mL of component A (AChE enzyme). The mixture was incubated for 15 minutes, after which 50 mL of component B was added to all the wells. Data were collected using a luminometer. Data shown represents t = 2.5 minutes after the addition of component B.