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Antibodies

Aurora B Rabbit monoclonal antibody

Product Sizes
20 ul
A19539-20UL
100 ul
A19539-100UL
500 ul
A19539-500UL
1000 ul
A19539-1000UL
About this Product
SKU:
A19539
Additional Names:
AIK2|AIM-1|AIM1|ARK-2|ARK2|AurB|aurkb-sv1|aurkb-sv2|Aurora B|IPL1|PPP1R48|STK-1|STK12|STK5
Application:
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Clonality:
Monoclonal
Host:
Rabbit
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Isotype:
IgG
Molecular Weight:
39kda
Purification:
Affinity Purified
Reactivities:
Human, Mouse, Rat
Sequence:
MAQKENSYPWPYGRQTAPSGLSTLPQRVLRKEPVTPSALVLMSRSNVQPTAAPGQKVMENSSGTPDILTRHFTIDDFEIGRPLGKGKFGNVYLAREKKSH
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Supplier:
ABclonal Technology
Type:
Antibody: Monoclonal Antibody
  • Manufacturer's Data Sheet:A19539
Western blot analysis of various lysates using Aurora B Rabbit mAb (A19539) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.
Confocal imaging of HeLa cells using Aurora B Rabbit mAb (A19539, dilution 1:800) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with A Alpha-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.