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Progen

Since 1983, PROGEN has been an established manufacturer and supplier of premium antibodies, in vitro diagnostics, and reagents for the global life science research community. The company’s portfolio attracts and serves a well-diversified clientele in research institutes and universities, pharmaceutical and biotech companies as well as private and clinical laboratories. While PROGEN ’s antibodies are among the most published antibodies in biomedical and cell biology literature, its ELISA kits aim at niche markets in microbiology, infectious diseases, and immunology. Building on its extensive core-competency and experience in immunochemistry, the company has expanded its product and service portfolio in recent years to include recombinant antibody engineering, antibody phage display technology, density gradient media, and AAV (adeno-associated virus) test kits for gene therapy research. Since October 2012, PROGEN has been a 100% subsidiary of the R-Biopharm AG. Together with the R-Biopharm Group and its partners PROGEN aims to support and extend existing markets as well as to enter new ones.

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Adeno-Associated Virus Antibodies and ELISA

Adeno-Associated Virus, AAV, a nonpathogenic virus species belonging to the Parvoviridae family. AAV is classified as small (25nm) containing a single-stranded nonenveloped DNA genome. Infection with AAV only occurs with the assistance of other viruses, for example herpesvirus or adenovirus (hence the name adeno-associated virus), causing only a very mild immune response in humans. There are twelve serotypes of human AAV but the number of nonhuman AAVs exceeds 100. AAV2 is the only mammalian DNA virus that is known to integrate in a specific site of the genome.

Immunogenicity is low and the ability to infect dividing as well as non-dividing cells with stable expression make the adeno-associated virus an appealing vector for the application in gene therapy. AAV has successfully been proven as gene therapy vector with the means to attach and enter the target cell, transfer to the nucleus and express the transgene in a stable manner over a sustained period of time. In several clinical trials (e.g. FIX, CFTR, Parkinsons’s, Canavan disease) AAV did not show any serious vector-related adverse effects. Clinically relevant tissues have, however, revealed a diversified susceptibility to AAV infection. The gene transduction with AAV2 vector in muscle, retina, liver and heart resulted in lower gene expression compared to AAV serotypes 1, 5, 8 and 9 transduction in the respective tissues. The challenge of using AAV as gene therapy vector, however, lies in the resistance of some tissues to transduction with the available AAV serotypes.

Target-Organs for AAV

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AAV, Adeno-Associated Virus, titration elisa, organs, progen
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