PROGEN AAV Workflow
Adeno-associated virus (AAV) is a powerful tool in gene therapy and biomedical research, requiring precise workflows to ensure reliable and reproducible results. 2BScientific Limited proudly distribute PROGEN’s comprehensive range of AAV reagents spanning ELISAs, antibodies, standards, Dip’n’Check tools, and OptiPrep resulting in a comprehensive solution for every stage of AAV analysis and quality control. These products seamlessly integrate to streamline AAV workflows, supporting researchers and developers in achieving consistent and accurate outcomes. Take a look at these reagents below and see how PROGEN can provide the essential tools needed to enhance your AAV research.
PROGEN's standard AAV ELISAs offer exceptional reliability for determining AAV capsid titres achieving a coefficient of variation (CV) below 10%. This relative high level of reproducibility makes them a reliable choice for researchers. Additionally, the assays are based on established international reference materials, such as ATCC standards for AAV2 and AAV8, ensure accurate and consistent quantification of intact AAV particles across experiments. The easy-to-use design of these kits simplifies workflows for all AAV serotypes, requiring no specialised equipment or training. Single-break strips allow researchers to perform multiple test runs efficiently.
AAV Xpress ELISAs
Additionally, PROGEN developed an Xpress version of their AAV ELISAs, which deliver reliable data in less than two hours. By optimising kit components and reducing assay time by over 50%, these ELISAs enable researchers to process a higher volume of samples without sacrificing accuracy or reproducibility.
Based on the same platform as the standard AAV ELISAs, the Xpress variants provide the same level of precision and reliability. This combination of speed and performance is particularly advantageous for laboratories managing high-throughput workflows.
AAV Antibodies
PROGEN offers a comprehensive portfolio of AAV antibodies, tailored to support every stage of AAV manufacturing and research. These high-quality reagents enable precise monitoring and optimisation of AAV related processes, ensuring reliability and consistency in gene therapy development. The range includes capsid protein antibodies for detecting denatured AAV proteins VP1, VP2, and VP3, and particle antibodies that recognise conformational epitopes unique to fully assembled capsids.
AAV Standards
PROGEN's fully characterised AAV Standards provide a reliable solution for ensuring data consistency across assays and studies. Each standard undergoes precise titre and molar concentration determination and meets stringent quality control protocols, ensuring accuracy and reproducibility. Ready-to-use and available off-the-shelf, these standards simplify workflows and minimise waiting times.
AAV Dip'n'Check
PROGEN's AAV Dip'n'Check lateral flow tests provide a fast and simple solution for semi-quantitative detection of fully assembled AAV capsids during development and manufacturing. Designed as a quick in-process control, these tests use PROGEN's exclusive AAV antibodies to deliver results in just 20 minutes. With an easy-to-follow protocol, they are suitable for both crude lysates and purified AAV samples, ensuring versatility across a range of workflows.
OptiPrep™
OptiPrep™ is a ready-to-use iodixanol solution for AAV particle purification and full/empty capsid separation. It’s a non-toxic, serotype-independent method offering high recovery rates and minimal damage to virus particles.
PROGEN AAV Reagent Catalogue | |