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IHC Kit Picker

Use this template below to choose your personalised IHC kit based on the species of your primary antibody.

KEY:


↓1) Select species primary antibody raised in                  
→2) Check options of species to be used on 
→3) Type of detection kit you'd like 
↓4) Check for extras! 

This is not an exhaustive list for ALL IHC! This list ONLY covers 'regular' Immunohistochemistry for Vector Laboratories. Xenograft tissues may require different treatment.

Multiple labelling can be possible, as can Immunofluorescence, Immunocytochemistry, PLA and others.

If you have any doubts or concerns, ask our in house IHC expert.

N.B. It is highly recommend to use IHC Validated antibodies where possible, e.g. MS Validated antibodies LSBio IHC Plus & PathPlus ranges. Generally, these are tested on FFPE, or Paraffin sections. Keep in mind that fixation can greatly affect the success of this method.

Try it for yourself!

Primary Antibody Raised in
Tissues / Cells to be used
1 step HRP and Substrate
(for substrate see below)
1 step AP and Substrate
(for substrate see below)
ABC-HRP and Substrate
(for substrate see below)
ABC-AP and Substrate
(for substrate see below)
Complete kit
(substrate included)
Complete, enhanced sensitivity kit
(substrate included)
RabbitOther than rabbitMP-7401

MP-7451
MP-5401 PK-6101 AK-5001 MP-7801 MP-7601 
 Veterinary species – Other than rabbitMP-6401      

       
MouseMouseMP-2400 PK-2200 BMK-2202 and AK-5000  
        
 RatMP-7422  BA-2001 and PK-6100BA-2001 and AK-5000  
        
 Other than mouse & ratMP-7402

MP-7452 
MP-5402 PK-6102 AK-5002 MP-7802 MP-7602 
        
 Veterinary species- Other than mouse & rodentMP-6402      








RatOnly mouseMP-7444 MP-5444 BA-4001 and PK-6100BA-4001 and AK-5000  
        
 Other than mouse & ratMP-7404 MP-5404 PK-6104 AK-5004   

       
GoatOther than bovine, ovine & caprineMP-7405 MP-5405 PK-6105 BA-5000 and AK-5000  

       
HumanOther than human  PK-6103 BA-3000 and AK-5000  
 Only humanHOH-3000      








Guinea pigOther than guinea pig  BA-7000 and PK-6100BA-7000 and AK-5000  








SheepOther than ovine, bovine & caprine  PK-6106 BA-6000 and AK-5000  








ChickenAll but chicken  BA-9010 and PK-6100 BA-9010 and AK-5000   








‘Universal’ Mouse OR RabbitOther than mouse, rabbit & ratMP-7500 PK-6200 AK-5200 MP-7800
PK-8200 
 
        
Double labelling kit for 1 Mouse AND 1 Rabbit antibodyOther than mouse, rabbit or ratMP-7714 MP-7724     
        
Further reagents required Substrate – e.g. SK-4105Substrate – e.g. SK-5105Substrate – e.g. SK-4105Substrate – e.g. SK-5105  
Further optional reagents  SP-2001 Avidin Biotin Blocking kit (Biotin methods only)  
Bloxall SP-6000

Antigen unmasking solutions H-3300 or H-3301

ImmEdge Pen H-4000


VectaMount Mounting Media H-5700 or H-5501 – select based on substrate requirements

For boxes with "and" Animal Free blocker (or serum) also required – Animal free blocker is generic alternative, sera must be selected to match the secondary.