IHC Kit Picker
Use this template below to choose your personalised IHC kit based on the species of your primary antibody.
KEY:
↓1) Select species primary antibody raised in | |
→2) Check options of species to be used on | |
→3) Type of detection kit you'd like | |
↓4) Check for extras! |
This is not an exhaustive list for ALL IHC! This list ONLY covers 'regular' Immunohistochemistry for Vector Laboratories. Xenograft tissues may require different treatment.
Multiple labelling can be possible, as can Immunofluorescence, Immunocytochemistry, PLA and others.
If you have any doubts or concerns, ask our in house IHC expert.
N.B. It is highly recommend to use IHC Validated antibodies where possible, e.g. MS Validated antibodies LSBio IHC Plus & PathPlus ranges. Generally, these are tested on FFPE, or Paraffin sections. Keep in mind that fixation can greatly affect the success of this method.
Try it for yourself!
Primary Antibody Raised in | Tissues / Cells to be used | 1 step HRP and Substrate (for substrate see below) | 1 step AP and Substrate (for substrate see below) | ABC-HRP and Substrate (for substrate see below) | ABC-AP and Substrate (for substrate see below) | Complete kit (substrate included) | Complete, enhanced sensitivity kit (substrate included) |
---|---|---|---|---|---|---|---|
Rabbit | Other than rabbit | MP-7401 MP-7451 | MP-5401 | PK-6101 | AK-5001 | MP-7801 | MP-7601 |
Veterinary species – Other than rabbit | MP-6401 | ||||||
Mouse | Mouse | MP-2400 | PK-2200 | BMK-2202 and AK-5000 | |||
Rat | MP-7422 | BA-2001 and PK-6100 | BA-2001 and AK-5000 | ||||
Other than mouse & rat | MP-7402 MP-7452 | MP-5402 | PK-6102 | AK-5002 | MP-7802 | MP-7602 | |
Veterinary species- Other than mouse & rodent | MP-6402 | ||||||
Rat | Only mouse | MP-7444 | MP-5444 | BA-4001 and PK-6100 | BA-4001 and AK-5000 | ||
Other than mouse & rat | MP-7404 | MP-5404 | PK-6104 | AK-5004 | |||
Goat | Other than bovine, ovine & caprine | MP-7405 | MP-5405 | PK-6105 | BA-5000 and AK-5000 | ||
Human | Other than human | PK-6103 | BA-3000 and AK-5000 | ||||
Only human | HOH-3000 | ||||||
Guinea pig | Other than guinea pig | BA-7000 and PK-6100 | BA-7000 and AK-5000 | ||||
Sheep | Other than ovine, bovine & caprine | PK-6106 | BA-6000 and AK-5000 | ||||
Chicken | All but chicken | BA-9010 and PK-6100 | BA-9010 and AK-5000 | ||||
‘Universal’ Mouse OR Rabbit | Other than mouse, rabbit & rat | MP-7500 | PK-6200 | AK-5200 | MP-7800 PK-8200 | ||
Double labelling kit for 1 Mouse AND 1 Rabbit antibody | Other than mouse, rabbit or rat | MP-7714 | MP-7724 | ||||
Further reagents required | Substrate – e.g. SK-4105 | Substrate – e.g. SK-5105 | Substrate – e.g. SK-4105 | Substrate – e.g. SK-5105 | |||
Further optional reagents | SP-2001 Avidin Biotin Blocking kit (Biotin methods only) | ||||||
Bloxall SP-6000 Antigen unmasking solutions H-3300 or H-3301 ImmEdge Pen H-4000 VectaMount Mounting Media H-5700 or H-5501 – select based on substrate requirements For boxes with "and" Animal Free blocker (or serum) also required – Animal free blocker is generic alternative, sera must be selected to match the secondary. |