Human Cytokine Products

Highly Active Human Interferon Proteins

High quality recombinant proteins derived from bacterial or mammalian cells

• Consistent performance and biological activity
• 95% or greater purity
• Endotoxin level < 1 EU/μg
• Suitable for use in a range of applications including ELISAs & cytopathic effect assays

Featured:
Universal Type I Interferon (Cat. No. 11200)
Unique hybrid interferon exhibiting activity across multiple species

• Useful for cross-species comparative studies of Type I IFN effects
• Broad crossover to multiple animal models in vivo and in vitro where the species relevant Type I IFN-α is unavailable or performs inconsistently
• High specific activity on multiple animal cell lines

Figure 1. Comparison of the Activities of Universal Type I IFN, Human IFN Alpha 2a, and Mouse IFN Alpha A

*IFNa-A (2a); IFNa-B2 (8); IFNa-C (10); IFNa-D (1 [Val(114)]); IFNa-F (21); IFNa-G (5); IFNa-H2 (14); IFNa-I (17); IFNa-J1 (7); IFNa-K (6); IFNa-4b (4); IFNa-WA (16)

To learn more, please contact Info@2BScientific.com or call us at +44 (0)1869 238033!

Human Interferon Alpha (IFN-α) ELISAs

Featured:
Human IFN Alpha All Subtype ELISA (Cat. No. 41115)
High Sensitivity

• Assay Standard Range: 1.95 – 125 pg/ml
• Accurate low pg/ml detection of all human IFN-α subtypes
• Robust matrix tolerance including autoimmune disease sera, normal serum, and tissue culture media (TCM)
• Optimized for autoimmune serum and plasma samples
• Consistent, reproducible results with < 10% inter- and < 8% intra-assay CVs
• Detects previously “missed” IFN-α subtypes

Table 1. Lower Limit of Quantitation (LLOQ) and Lower Limit of Detection (LLOD) in Normal Human Serum.

Figure 2. IFN-α Specific Signal in Lupus Patients. Lupus patient serum number. Purple bars represent non-specific signal, i.e., signal not blocked by AntiIFN-α PAb.

To learn more, please contact Info@2BScientific.com or call us at +44 (0)1869 238033!

Human Interferon Beta (IFN-β) ELISAs

Featured:
Human IFN Beta Serum ELISA (Cat. No. 41415)
High Sensitivity

• Assay Standard Range: 1.2 – 150 pg/ml
• Provides > 90% serum spike-recovery
• Reproducible results with < 8% inter- and intra-assay CVs
• Robust matrix tolerance for autoimmune disease sera
• Validated by multiple CROs for sensitive measurement of IFN-β therapeutics in human serum samples
• e.g. Rebif®, Avonex®, Extavia®, Betaseron®
• Suitable for PK, PD, Toxicity & Biomarker studies

Figure 3. ELISA Standard Curve in Normal Human Serum (NHS) vs. Standard Curve in Standard Diluent. No observable inhibitory effect of normal human serum.

To learn more, please contact Info@2BScientific.com or call us at +44 (0)1869 238033!

Anti-Human IFN Monoclonal (MAb) & Polyclonal (PAb) Antibodies

Labeled & unlabeled antibodies for a variety of uses

• MAbs offer high specificity in applications where specific binding is desirable
• PAbs are preferable for neutralization because they target a variety of specific epitopes of a particular antigen

Featured: Anti-Human IFNAR2, Clone MMHAR-2 (MAb) (Cat. No. 21385-1)

• Blocks biological activity of human Type I IFNs and IFN-α receptor
• Binds to human IFN-α receptor with high affinity

Human Type I IFN Neutralizing Antibody Mixture (Cat. No. 39000)
Mixture of MAbs and PAbs directed against human Type I IFNs & IFNAR2

• Effectively neutralizes biological activity of human IFN-α, IFN-β, IFN-ω, IFN-κ, and IFN-ε
• Improves neutralization of multiple Type I IFNs often found in complex samples by employing a broadly neutralizing custom antibody cocktail

Figure 5. Mouse MAb to Human IFN-Alpha/Beta Receptor 2.

Figure 6. Representative neutralization curves (diluted 1:50) to human IFN-α, -β, and -ω. A549 cells incbated with antibody mixture and titrated IFN, subsequently challenged with EMCV.