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Antibodies

DARPP-32 Phospho T34 Antibody

Product Sizes
100 ul
612-401-C73-100UL
About this Product
SKU:
612-401-C73
Additional Names:
Ppp1r1b|Protein phosphatase 1 regulatory subunit 1B, Dopamine- and cAMP-regulated neuronal phosphoprotein
Application:
ELISA, Western Blot
Buffer:
0.01 M HEPES, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Extra Details:
DARPP-32 Antibody detects DARPP-32 which is a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons. The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA. Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac®. Anti-DARPP-32 pT34 Antibody is ideal for investigators involved in Neuroscience and Cell Signaling Research.
Formulation:
0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Host:
Rabbit
Immunogen:
Anti-DARPP 32 pT34 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Thr34 conjugated to KLH.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Rat
Shipping Conditions:
Dry Ice
Specificity:
Anti-DARPP-32 pT34 antibody is directed against DARPP-32. Anti-DARPP-32 pT34 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin ar
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot of Rabbit anti-DARPP-32 Thr34 Antibody. Lane 1: rat caudate lysate. Lane 2: rat caudate lysate incubated in L Lambda-Ptase (1200 units for 30 min). Load: 10 ug per lane. Primary antibody: DARPP-32 Thr34 antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~32kDa/~32kDa for DARPP-32 phosphorylated at Thr34. Other band(s): none.