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Antibodies

BIF Antibody

Product Sizes
100 ug
600-401-Z67-100UG
About this Product
SKU:
600-401-Z67
Additional Names:
Bif Antibody, Bif-1, CGI-61, PPP1R70, dJ612B15.2, KIAA0491, Endophilin-B1, Bax-interacting factor 1, Bif-1|SH3GLB1
Application:
ELISA, Immunohistochemistry, Western Blot
Buffer:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by the activation of proteolytic enzymes termed caspases. Proteins that comprise the Bcl-2 family such as Bax appear to control the activation of these enzymes. Bax activity was found to be regulated by its association with Bax-interacting factor 1 (BIF), a member of the endophilin B family that is associated with intracellular membranes. Following this interaction, Bax undergoes a conformational change and translocates to mitochondrial membranes. The Bax/BIF interaction appears to be enhanced by apoptotic stimuli, suggesting that BIF acts as the trigger to activate Bax, and as suppression of BIF promoted HeLa cell colony formation in soft agar, it may have a role in the suppression of cancer progression. At least two isoforms of BIF are known to exist.
Formulation:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-BIF antibody was prepared from whole rabbit serum produced by repeated immunizations with a 15 amino acid synthetic peptide from near the C-terminus of human BIF.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human, Mouse
Shipping Conditions:
Dry Ice
Specificity:
Anti-Bif Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Cross reactivity with Bif from other sources has not been determined.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot of BIF antibody in HeLa cell lysate. Lane A: BIF antibody at 1 ug/mL. Lane B: BIF antibody at 2 ug/mL. Load: 35 ug per lane. Primary antibody: BIF antibody at designated concentrations for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 41 kDa, 38 kDa for BIF. Other band(s): BIF splice variants and isoforms.
Immunocytochemistry of BIF antibody. Cell Type: HeLa cells. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: BIF antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: BIF is located in the cytoplasm, golgi apparatus, cell membrane, mitochondrion, and mitochondrion outer membrane. Staining: BIF is stained brown with hematoxylin purple nuclear counterstain.