Skip to content

Basket

You currently have no items in your basket.

Total (excl. vat) £0.00
View basket & checkout
Antibodies

ABIN3 Antibody

Product Sizes
100 ug
600-401-X57-100UG
About this Product
SKU:
600-401-X57
Additional Names:
ABIN3 Antibody, LIND, ABIN-3, ABIN3, LIND, TNFAIP3-interacting protein 3, A20-binding inhibitor of NF-kappa-B activation 3|TNIP3
Application:
ELISA, Immunohistochemistry, Western Blot
Buffer:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
The nuclear factor NF-kB plays key roles in development and immunity. ABIN3 (A20-binding inhibitor of NF-kB activation 3), also known as TNFAIP3-interacting protein 3 (TNIP3), is a novel negative feedback regulator of LPS-induced NF-kB activation. ABIN3 is a 39 kDa protein that negatively regulates NF-kB activation in response to TNF and LPS. ABIN3 is highly expressed in brain, thymus, lymph node, lung and fetal liver, with low expression in kidney, bone marrow. Through its interaction with A20, ABIN3 interferes with TRAF2-mediated transactivation signals and NF-kB inhibition is mediated by the ABIN-homology domain 2. ABIN3 has been found to be induced by Listeria infection and can be slightly downregulated by dexamethasone. Enhanced expression of ABIN3 in monocytes is associated with sepsis. Thus, ABIN3 is an IL-10-induced gene product capable of attenuating NF-kB in human macrophages yet is inoperative in mice and represents a basis for species-specific differences in IL-10 actions. At least four isoforms of ABIN3 are known to exist.
Formulation:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-ABIN3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 17 amino acid synthetic peptide near the C-terminus of human ABIN3.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human, Mouse
Shipping Conditions:
Dry Ice
Specificity:
Anti-ABIN3 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Cross reactivity with ABIN3 from other sources has not been determined.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot of ABIN3 antibody. Lane 1: human spleen tissue lysate in the absence of blocking peptide. Lane 2: human spleen tissue lysate in the presence of blocking peptide. Primary Antibody: Anti-ABIN3 antibody at 1 ug/mL. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 39 kDa, 36 kDa for ABIN3. Other band(s): ABIN3 splice variants and isoforms.
Immunohistochemistry of ABIN3 antibody. Tissue: mouse spleen tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: ABIN3 antibody at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: ABIN3 as precipitated pink signal with blue nuclear counterstain.