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Antibodies

BCL3 Antibody

Product Sizes
25 ul
600-401-GU4S-25UL
About this Product
SKU:
600-401-GU4S
Additional Names:
BCL3|rabbit anti-BCL3 antibody, rabbit anti-BCL-3 Antibody, BCL 3, B-cell lymphoma 3 protein, B-cell lymphoma 3 protein Antibody
Application:
ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
BCL3 belongs to the ANKRD family. The protein contains seven ankyrin repeats, which are closely related to those found in I kappa B proteins. BCL3 functions as a transcriptional co-activator, regulator of transcriptional activation, promotor of transcription, and inhibitor of nuclear translocation of NF-kappa B. It is a proto-oncogene identified by its translocation into the immunoglobulin alpha-locus in some cases of B-cell leukemia. BCL3 contributes to the regulation of cell proliferation. Therefore, BCL3 may be associated with B-cell chronic lymphocytic leukemia, lymphoma, or other hematologic diseases. Anti-BCL3 Antibody is useful for researchers interested in Cancer Research, NF-Kappa B Pathway, and TNF Signaling Pathway.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-BCL3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Term portion of human BCL3 conjugated to Keyhole Limpet Hemocyanin (KLH).
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human
Shipping Conditions:
Dry Ice
Specificity:
This affinity purified antibody is directed against human BCL3. This product was affinity purified from monospecific antiserum by immunoaffinity purification. Blast analysis of the sequence of the immunogen shows 100% identity with human.
Storage Conditions:
-20[o]C diluted. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Immunofluorescence microscopy of BCL3 in Caco-2 cells using FITC-conjugated Fluorescent TrueBlot® anti-rabbit IgG for detection. Caco-2 cells were fixed with 4% PFA, blocked (5% mouse serum/0.3% Triton X-100 in 1X PBS ) for 1 hr, then incubated with 15 ug/mL of anti-BCL3 primary antibody (Cat. No. 600-401-GU4) at 4°C overnight. Following 3 washes in 1X PBS for 5 min each, 5 ug/mL of FITC-conjugated Fluorescent TrueBlot® anti-rabbit IgG was added and allowed to incubate for 1 hr at room temperature. Nuclei were counterstained with DAPI present in mounting medium. The predicted main localization is nucleoplasm. Additional localization in some cell types includes vesicles and midbody. (a) BCL3 (b) DAPI (c) merged DAPI/BCL3 (d) secondary antibody only. Image taken at 40X magnification.
Western Blot of Rabbit anti-BCL3 antibody. Lane 1: MW ladder (p/n MB-210-0500). Lane 2: HEK293T WCL. Lane 3: BCL3 overexpressing HEK293T lysate. Load: 10 ug per lane. Primary antibody: BCL3 antibody at 1:1000 for overnight at 4°C. Secondary antibody: goat anti-rabbit secondary Antibody DyLight™ 488 antibody (p/n 611-141-122) at 1:20,000 for one hour at RT. Block: BlockOut blocking buffer (p/n MB-073) one hour at RT. Predicted/Observed size: 48 kda. Other band(s): BCL3 processing caused by ubiquitination, phosphorylations.
Western Blot of Rabbit anti-BCL3 antibody. Lane 1: MW ladder (p/n MB-210-0500). Lane 2: U-87 MG WCL. Lane 3: C2C12 WCL (p/n W10-001-GL7). Lane 4: NIH/3T3 WCL (p/n W10-000-358). Load: 10 ug per lane. Primary antibody: BCL3 antibody at 1:1000 for overnight at 4°C. Secondary antibody: goat anti-rabbit secondary HRP antibody (p/n 611-103-122) at 1:70,000 for one hour at RT. Block: 1% Casein (p/n MB-081-1000) (PVDF) blocking buffer one hour at RT. Predicted/Observed size: 47 kda. Other band(s): BCL3 processing caused by ubiquitination, phosphorylations.
Immunofluorescence Microscopy of Rabbit anti-BCL3 antibody. Tissue: U-87-MG cells. Fixation: 4% PFA. Antigen retrieval: not required. Primary antibody: BCL3 antibody at 10 ug/mL overnight at 4⁰C. Secondary antibody: Donkey Anti-Rabbit IgG DyLight™ 488 (p/n 611-741-127) at 5 ug/ml for 2 h at RT. Localization: BCL3 is subcellularly localized in the cytosol, nucleoplasm, midbody and vesicles. Staining: BCL3 as green fluorescent signal with DAPI (blue) nuclear counterstain.