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Antibodies

NAIP Antibody

Product Sizes
100 ug
600-401-CX3-100UG
About this Product
SKU:
600-401-CX3
Additional Names:
NAIP|NAIP Antibody, BIRC1, NLRB1, psiNAIP, BIRC1, Baculoviral IAP repeat-containing protein 1, Neuronal apoptosis inhibitory protein
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Neuronal apoptosis inhibitor protein (NAIP) was the first human inhibitor of apoptosis protein (IAP) identified and was discovered by its association with the neurodegenerative disorder spinal muscular atrophy. Members of the IAP family contain one to three copies of an approximately 70 amino acid motif termed baculovirus IAP repeat (BIR); these BIRs promote protein-protein interactions with various caspases such as caspase-3, -7, and -9 as well as members of the TRAF family of signal molecules. Unlike other IAPs however, NAIP requires ATP to bind caspase-9 and is not inhibited by the IAP-inhibiting molecule Smac/DIABLO, suggesting that NAIP is unique among the IAPs in its regulation of its activity. Finally, although only one human NAIP protein has been identified, other shorter NAIP mRNA transcripts have been reported.
Formulation:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-NAIP antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to 13 amino acids at the C-terminus of human NAIP.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human
Shipping Conditions:
Dry Ice
Specificity:
Anti-NAIP Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Minor, lower molecular weight bands may represent alternately spliced forms.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Immunocytochemistry of NAIP antibody. Tissue: A549 cells. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: NAIP antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: NAIP is located in the basolateral plasma membrane, cytoplasm, extracellular exosome, and nucleus. Staining: NAIP is stained brown with hematoxylin purple counterstain.
Immunofluorescence Microscopy of NAIP antibody. Cell Type: A549 cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: NAIP antibody at 20 ug/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: NAIP is located in the basolateral plasma membrane, cytoplasm, extracellular exosome, and nucleus. Staining: NAIP as red fluorescent signal.
Western Blot of NAIP antibody in PC-3 cell lysate. Lane A: NAIP antibody at 0.5 ug/mL. Lane B: NAIP antibody at 1 ug/mL. Lane C: NAIP antibody at 2 ug/mL. Load: 35 ug per lane. Primary antibody: NAIP antibody at designated concentrations for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 160 kDa, 140 kDa for NAIP. Other band(s): NAIP splice variants and isoforms.