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Antibodies

Cyclin L1/L2 Antibody

Product Sizes
500 ug
200-401-890-500UG
About this Product
SKU:
200-401-890
Additional Names:
CCNL1, CCNL2|rabbit anti-Cyclin L1/L2 Antibody, rabbit anti-Cyclin L1 Antibody, rabbit anti-Cyclin L2 Antibody, Cyclin L beta, CCNL1, CCNL2, Paneth cell-enhanced expression protein
Application:
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
5 mg/ml
Extra Details:
Cyclin L (also referred to as CCNL) is encoded by two highly related genes Cyclin L1 and Cyclin L2 (CCNL1 and CCNL2, respectively). Cyclin L has been shown to associate with the PITSLRE kinase and is involved in pre-mRNA processing. Both Cyclin L gene products can be alternatively spliced to produce two isoforms (known as 1 and 2 or alpha and beta). Cyclin L2 is ubiquitous, expressed at much higher levels than Cyclin L1, and thus is likely the major partner for the CDK11p110 protein kinase.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic construct consisting of full length Human Cyclin L1b protein.
Isotype:
IgG
Physical State:
Lyophilized
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Ambient
Specificity:
The product was purified from monospecific antiserum by Protein A chromatography. This antibody will react with all forms of Cyclin L b (cyclin L1b and cyclin L2b). Cross reactivity with Cyclin L beta proteins from other sources is unknown.
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Rockland's Affinity Purified anti- Cyclin L1/L2 (isoform 2) antibody was used at a 10 ug/ml to detect cyclin L in a variety of tissues including breast, kidney, liver, lung, skeletal muscle, pancreas, prostate and spleen. In some tissues elevated background staining was noted. In these instances further optimization of dilution is suggested. This image shows Cyclin L staining of human skeletal muscle. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.
Western blot of Anti-Cyclin L1/L2B Beta antibody. Lane 1: mouse brain whole cell lysate (p/n W10-000-T004) [35ug] (800 nm channel - green). Marker proteins appear red (700 nm channel). Primary Antibody: Anti-Cyclin L1/L2B Beta at 1:2,500 for 2h at room temperature. Secondary Antibody: 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX (p/n 611-132-122) for 45 min at room temperature. Block: (p/n MB-070) Buffer. Imaging: captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.