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Antibodies

Alcohol Dehydrogenase Antibody

Product Sizes
2 ml
100-4143-2ML
About this Product
SKU:
100-4143
Additional Names:
ADH1|rabbit anti-Alcohol Dehydrogenase Antibody, Alcohol dehydrogenase 1, Alcohol dehydrogenase I, YADH-1
Application:
ELISA, Immunoprecipitation, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
90 mg/ml
Extra Details:
Alcohol dehydrogenase is an isozyme that preferentially catalyzes the conversion of acetaldehyde to acetone. Alcohol dehydrogenase has an apparent molecular weight of 37 kDa (monomer subunit) and forms a homotetramer. This enzyme acts on a variety of primary unbranched aliphatic alcohols and requires 2 bound zinc ions per subunit. Alcohol dehydrogenase shows a cytoplasmic localization. Microheterogeneities may also occur at positions 137, 138, 242-244, and 255 and near position 287.
Format:
Antiserum
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
This antibody was prepared from whole rabbit serum produced by repeated immunizations with full length Alcohol Dehydrogenase isolated from yeast.
Physical State:
Lyophilized
Reactivities:
Yeast/Fungi
Shipping Conditions:
Ambient
Specificity:
This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Alcohol Dehydrogenase [Yeast]. Cross reactivity against Alcohol Dehydrogenase from most fungal sources is likely due to sequence homology as determined by BLAST analysis. Cross reacti
Storage Conditions:
2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot of Anti-Alcohol Dehydrogenase Antibody. High pressure promotes Sch9 phosphorylation in an EGOC- and Pib2-dependent manner. (B) Whole cell lysates were prepared from the rrd1D Deltasch9D Delta mutant expressing wild-type (WT) or point-mutated Sch9-3HA (CEN) and analyzed by western blotting using anti-HA and anti-Adh1 antibodies. OD600 from more than three independent experiments. Protein levels in B were quantified using ImageJ software and are presented as mean+/-s.d. from three independent experiments. Fig 4. PMID: 32801125.
Western blot analysis with Rockland's Anti-Alcohol Dehydrogenase antibody was used to detect yeast Alcohol Dehydrogenase. Comparison to molecular weight markers (not shown) indicates estimated molecular weights consistent with monomer, dimer and tetramer present in this preparation. The blot was incubated with a 1:500 dilution of the antibody at room temperature for 2 h followed by detection using IRDye™800 labeled Goat-a-Rabbit IgG [H&L] (611-132-122) diluted 1:5,000 for 45 min at room temperature. The IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.