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Antibodies

Rabbit Anti-Phospho-CaMKII (T286) Antibody [JE51-81], Recombinant Rabbit monoclonal Antibody [NeuroAb]

Product Sizes
100uL
HA721794-HA721794
About this Product
SKU:
HA721794
Application:
WB
Buffer:
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
JE51-81
Clonality:
Recombinant Rabbit monoclonal Antibody
Concentration:
1 mg/mL.
Control:
Human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate.
Format:
Liquid
Host:
Rabbit
Immunogen:
Synthetic phospho-peptide corresponding to residues surrounding Thr286 of human CaMKII.
Isotype:
IgG
Molecular Weight:
Predicted band size: 54 kDa
Purification:
Protein A affinity purified.
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Western blot analysis of Phospho-CaMKII (T286) on different lysates with Rabbit anti-Phospho-CaMKII (T286) antibody (HA721794) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Mouse brain treated with λpp for 1 hour tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 54~73 kDa Observed band size: 50~60 kDa Exposure time: 53 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721794) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.