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Antibodies

Rabbit Anti-Phospho-ATR (T1989) Antibody [PS01-18], Recombinant Rabbit monoclonal Antibody

Product Sizes
100uL
HA721190-HA721190
About this Product
SKU:
HA721190
Application:
WB, IF-Cell
Buffer:
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
PS01-18
Clonality:
Recombinant Rabbit monoclonal Antibody
Concentration:
1 mg/ml
Control:
HeLa cell lysate, HeLa treated with Hydroxyurea cell lysate, HeLa treated with 4mM Hydroxyurea for 2
Format:
Liquid
Host:
Rabbit
Immunogen:
Synthetic peptide.
Isotype:
IgG
Molecular Weight:
Predicted band size: 301 kDa
Purification:
Protein A affinity purified.
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Western blot analysis of Phospho-ATR (T1989) on different lysates with Rabbit anti-Phospho-ATR (T1989) antibody (HA721190) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with Hydroxyurea cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721190) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of HeLa cells treated with or without 4mM Hydroxyurea for 20 hours labeling Phospho-ATR (T1989) with Rabbit anti-Phospho-ATR (T1989) antibody (HA721190) at 1/200 dilution. Image shown an increased nuclear staining upon Hydroxyurea treatment. Cells were fixed in 4% paraformaldehyde for 20 minutes at 37 ℃, permeabilized with 0.1% Triton X-100 in PBS permeabilization for 5 minutes, and then blocked with 2% negative goat serum for 60 minutes at room temperature. Cells were then incubated with Rabbit anti-Phospho-ATR (T1989) antibody (HA721190) at 1/200 dilution in 1% BSA overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor[TM] 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.