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Antibodies

Rabbit Anti-Apg3 Antibody [JU00-35], Recombinant Rabbit monoclonal Antibody [NeuroAb]

Product Sizes
100uL
ET1706-29-ET1706-29
About this Product
SKU:
ET1706-29
Application:
WB, IHC-Paraffin
Buffer:
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
JU00-35
Clonality:
Recombinant Rabbit monoclonal Antibody
Concentration:
1 mg/mL.
Control:
K562 cell lysate, HL-60 cell lysate, Hela cell lysate, Jurkat cell lysate, human tonsil tissue, huma
Format:
Liquid
Host:
Rabbit
Immunogen:
Synthetic peptide within Human Apg3 1-50 / 314.
Isotype:
IgG
Molecular Weight:
40 kDa
Purification:
Protein A affinity purified.
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Western blot analysis of Apg3 on different lysates with Rabbit anti-Apg3 antibody (ET1706-29) at 1/500 dilution. Lane 1: Mouse testis tissue(20 µg/Lane) Lane 2: K562 cell lysate Lane 3: HL-60 cell lysate Lane 4: Hela cell lysate Lane 5: Jurkat cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-29) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 5,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Apg3 antibody (ET1706-29) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-29) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue with Rabbit anti-Apg3 antibody (ET1706-29) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-29) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Apg3 antibody (ET1706-29) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-29) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Rabbit anti-Apg3 antibody (ET1706-29) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-29) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.