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Antibodies

Rabbit Anti-IP3 Receptor Antibody [JA11-35], Recombinant Rabbit monoclonal Antibody [NeuroAb]

Product Sizes
100uL
ET1704-77-ET1704-77
About this Product
SKU:
ET1704-77
Application:
WB, IHC-Paraffin
Buffer:
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
JA11-35
Clonality:
Recombinant Rabbit monoclonal Antibody
Concentration:
1 mg/mL.
Control:
Rat brain tissue lysate, mouse brain tissue lysate, rat cerebellum tissue, mouse cerebellum tissue.
Format:
Liquid
Host:
Rabbit
Immunogen:
Recombinant protein within Human IP3 Receptor aa 2190-2280 / 2758.
Isotype:
IgG
Molecular Weight:
Predicted band size: 314 kDa
Purification:
Protein A affinity purified.
Reactivities:
Mouse, Rat, Human
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Western blot analysis of IP3 Receptor on different lysates with Rabbit anti-IP3 Receptor antibody (ET1704-77) at 1/500 dilution. Lane 1: Rat brain tissue lysate Lane 2: Mouse brain tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 314 kDa Observed band size: 314 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-77) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-IP3 Receptor antibody (ET1704-77) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-77) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-IP3 Receptor antibody (ET1704-77) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-77) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.