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Antibodies

Rabbit Anti-Phospho-Histone H2A.X(S139) Antibody [SR33-09], Recombinant Rabbit monoclonal Antibody

Product Sizes
100uL
ET1602-2-ET1602-2
About this Product
SKU:
ET1602-2
Application:
WB, IHC-Paraffin
Buffer:
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
SR33-09
Clonality:
Recombinant Rabbit monoclonal Antibody
Concentration:
1 mg/mL.
Control:
HeLa treated with 20μM Etoposide for 2 hours whole cell lysate, HeLa treated with UV for 2 hours who
Format:
Liquid
Host:
Rabbit
Immunogen:
Synthetic phospho-peptide corresponding to residues surrounding Ser139 of Human Histone H2AX.
Isotype:
IgG
Molecular Weight:
Predicted band size: 15 kDa
Purification:
Protein A affinity purified.
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Storage Conditions:
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Supplier:
Huabio
Western blot analysis of Phospho-Histone H2A.X(S139) on different lysates with Rabbit anti-Phospho-Histone H2A.X(S139) antibody (ET1602-2) at 1/2,000 dilution. Lane 1: HeLa whole cell lysate Lane 2: HeLa treated with 20μM Etoposide for 2 hours whole cell lysate Lane 3: HeLa whole cell lysate Lane 4: HeLa treated with UV for 2 hours whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lane 6: NIH/3T3 treated with 25μM Etoposide for 5 hours whole cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 15 kDa Observed band size: 15/20 kDa Exposure time: 53 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-2) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-Histone H2A.X(S139) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-2, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate–treated with etoposide Lane 2: HepG2 cell lysate–untreated
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Phospho-Histone H2A.X(S139) antibody (ET1602-2) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-2) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Phospho-Histone H2A.X(S139) antibody (ET1602-2) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-2) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Phospho-Histone H2A.X(S139) antibody (ET1602-2) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-2) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.