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Antibodies

GABA A Receptor Alpha-1 Antibody, Clone N95/35: ATTO 488

Product Sizes
100 ug
SMC-346D-A488-100UG
About this Product
SKU:
SMC-346D-A488
Additional Names:
ECA4 antibody, EJM antibody, EJM5 antibody, Gaba receptor alpha 1 polypeptide antibody, GABA(A) receptor antibody, GABA(A) receptor subunit alpha 1 antibody, GABA(A) receptor subunit alpha-1 antibody, GABA(A) receptor, alpha 1 antibody, GABRA 1 antibody, GABRA1 antibody, Gamma aminobutyric acid (GABA) A receptor alpha 1 antibody, Gamma aminobutyric acid A receptor alpha 1 antibody, Gamma aminobutyric acid receptor subunit alpha 1 antibody, Gamma-aminobutyric acid receptor subunit alpha-1 antibody, GBRA1_HUMAN antibody
Application:
Antibody Microarray, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
PBS, 50% glycerol, 0.1% azide *Storage buffer may change when conjugated
CE/IVD:
RUO
translate.label.attr.clone:
N95/35 (Formerly sold as S95-35)
Clonality:
Monoclonal
Conjugate:
ATTO 488
Concentration:
1 mg/ml
Extra Details:
The GABA-A receptor is a member of the superfamily of fast acting ligand-gated ion channels. The individual subunits of these receptors have similar sequences and structural features (1). GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain (2).
Host:
Mouse
Immunogen:
Fusion protein amino acids 355-394 of human GABA-A-R-Alpha1
Isotype:
IgG2a
Purification:
Protein G Purified
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
Detects ~55kDa. No cross-reactivity against GABA-A-R-Alpha 2 or -Alpha3.
Storage Conditions:
See Manual
Supplier:
StressMarq Biosciences
Type:
Antibody: Monoclonal Antibody
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-A Receptor Alpha-1 Monoclonal Antibody, Clone N95/35 (SMC-346). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA-A Receptor Alpha-1 Monoclonal Antibody (SMC-346) at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-A Receptor Alpha-1 Antibody (D) Composite.
Immunohistochemistry analysis using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone N95/35 (SMC-346). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody (SMC-346) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dermal Cells.
Western Blot analysis of Human Cell line lysates showing detection of GABA A Receptor protein using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone N95/35 (SMC-346). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody (SMC-346) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.