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Antibodies

GABAA Receptor beta 3 Antibody

Product Sizes
100 ul
600-401-C79-100UL
About this Product
SKU:
600-401-C79
Additional Names:
Gabrb3|Gamma-aminobutyric acid receptor subunit beta-3, GABA(A) receptor subunit beta-3, Gabrb3
Application:
Detection/Quantitation, Immunohistochemistry, Western Blot
Buffer:
0.01 M HEPES, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Extra Details:
Anti-GABA(A) Receptor beta 3 Antibody detects GABA(A) Receptor beta 3. Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six A Alphas, four B Betas and four G Gammas, plus alternative splicing variants of some of these subunits, have been identified. Injection in oocytes or mammalian cell lines of cRNA coding for A Alpha- and B Beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a G Gamma-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different A Alpha-subunits of the receptor. GABA(A) receptor beta 3 antibody is ideal for investigators involved in Neuroscience.
Formulation:
0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Host:
Rabbit
Immunogen:
Anti-GABA(A) Receptor beta 3 Antibody was produced by repeated immunizations with recombinant fusion protein from the cytoplasmic loop of the beta 3 subunit of rat GABAA.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Mouse, Rat
Shipping Conditions:
Dry Ice
Specificity:
Anti-GABA(A) Receptor beta 3 Antibody is directed against rat GABA(A) Receptor beta 3. The antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Reactivity is expected from the following species based on 100% sequence homology: mouse. Cross reactivity with GABA(A) Receptor beta 3 from other species has not been determined.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Immunohistochemistry of Anti-GABA(A) Receptor beta 3 (Rabbit) Antibody. Tissue: mouse retina. Labeling: GABA(A) Receptor beta 3 subunit in green, calbindin in red, and DNA in blue.
Western Blot of Anti-GABA(A) Receptor beta 3 (Rabbit) Antibody. Lane 1: rat brain lysate. Primary Antibody: 1ug/mL of Anti-GABA(A) Receptor beta 3. Secondary Antibody: Goat Anti-Rabbit HRP of 611-103-122 at 1:40,000. Block: Universal BlockOut buffer MB-073. Expect: ~53 kDa.
Western Blot of Rabbit anti-GABAA Receptor B Beta3 Antibody. Lane 1: mouse cerebellum lysates from wild type. Lane 2: mouse cerebellum lysates from B Beta3 knockout (B Beta3 K/O). Load: 5-7 ug per lane. Primary antibody: GABAA-R antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~53kDa/~53kDa for B Beta3-subunit of the GABAA-R in the wild type. Other band(s): none.