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Antibodies

TNF alpha Antibody

Product Sizes
100 ul
209-401-306-0100-100UL
About this Product
SKU:
209-401-306-0100
Additional Names:
APC1 antibody, Cachectin antibody, DIF antibody, Differentiation inducing factor antibody, Macrophage cytotoxic factor antibody, MCF antibody, Necrosin antibody, Tumour Necrosis Factor Alpha antibody, rabbit anti-Tumor Necrosis Factor Alpha Antibody, rabbit anti-TNF Alpha Antibody, Tumor necrosis factor, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Tumor necrosis factor membrane form, N-terminal fragment, NTF, Intracellular domain 1, ICD1, Intracellular domain 2, ICD2, C-domain 1, C-domain 2, Tumor necrosis factor, soluble form, TNF, TNFA, TNFSF2|TNF
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Anti TNF alpha Antibody recognizes TNF alpha (TNF, cachexin, cachectin, tumor necrosis factor-alpha or TNF-A Alpha) a cytokine involved in systemic inflammation. TNF alpha is a member of a group of cytokines that stimulate the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by other cell types as well. The primary role of TNF alpha is in the regulation of immune cells. TNFis an endogenous pyrogen that is able to induce fever, apoptotic cell death, sepsis (through IL-1 & IL-6 production), cachexia, inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNF production has been implicated in a variety of human diseases, including Alzheimer's disease, cancer, major depression, and inflammatory bowel disease (IBD).
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
TNF alpha antibody used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human TNFA Alpha.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human
Shipping Conditions:
Dry Ice
Specificity:
This antibody is primarily directed against mature 17,000 MW human TNFA Alpha and is useful in determining its presence in various assays. In general, this antibody also detects primate TNFA Alpha in the same formats using similar dilutions. The antibody does not recognize human TNFB Beta (lymphotoxin). This IgG fraction antibody will recognize the cell-bound precursor of TNFA Alpha as a 26,000 pr
Storage Conditions:
2-8[o]C Aliquot. Avoid freeze/thaw cycles. Store undiluted.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot of Rabbit anti-TNF Alpha antibody. Marker: Opal Pre-stained ladder (p/n MB-210-0500). Lane 1: HEK293 lysate (p/n W09-000-365). Lane 2: HeLa Lysate (p/n W09-000-364). Lane 3: MCF-7 Lysate (p/n W09-000-360). Lane 4: Jurkat Lysate (p/n W09-000-370). Lane 5: A431 Lysate (p/n W09-000-361). Lane 6: A549 Lysate (p/n W09-001-372). Lane 7: LNCap Lysate (p/n W09-001-GJ9). Lane 8: MOLT-4 Lysate (p/n W09-001-GK2). Lane 9: Ramos Lysate (p/n W09-000-GK4). Lane 10: Raji Lysate (p/n W09-001-368). Lane 11: A-172 Lysate (p/n W09-001-GL5). Lane 12: NIH/3T3 Lysate (p/n W10-000-358). Load: 35 ug per lane. Primary antibody: TNF Alpha antibody at 1ug/mL overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n 611-103-122) at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS (p/n MB-082) for 30 min at RT. Predicted/Observed size: 26kDa for TNF Alpha.
(I) Immunostaining for TNF-a: (A) negative control; (B) muscle from subject 465 yr (positive control); (C) muscle from weight maintainer; and (D) muscle from weight gainer. Anti-human TNF-a antibody (p/n 209-401-306) at 1:50. The sites of peroxidase binding were demonstrated with diamonobenzidine (p/n DAB-50). Fig 2. PMID: 16687193.
Fluorescent immunohistochemistry showing staining of human colon by Rockland's anti-TNF alpha (formalin/PFA-fixed paraffin-embedded sections). Samples were formaldehyde-fixed, then blocked in 10% serum for 2 hours at 20°C. The primary antibody was diluted 1:100 and incubated with the sample for 2 hours at 20°C. Alexa Fluor® 680 goat polyclonal secondary antibody was used diluted 1:5000.
Immunohistochemistry using Rockland's polyclonal TNFa antibody showing staining of formalin/PFA-fixed paraffin-embedded sections of human artery tissue sections. Sections were fixed in formaldehyde and subjected to heat mediated antigen retrieval in citrate buffer (pH 6.0). Slides were blocked for ten minutes with 1.5% serum. Primary antibody was diluted 1:100 and incubated with samples for 24 hours at 4°C. HRP-conjugated goat anti-rabbit antibody was used as the secondary antibody.
Western blot using Rockland's Anti-Human TNF-a (RABBIT) Antibody. Membrane blocked in 1% BSA-TBS-T for 30 min at RT, Rb-a-TNF alpha added at 1:1000 in 1% BSA-TBS-T o/n 4°C, DyLight 649 Gt-a-Rb (p/n 611-143-122) added at 1:20,000 in buffer (p/n MB-070) for 30 min at RT.