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Antibodies

Tau Antibody (pThr217), Clone 15B7: APC

Product Sizes
100 ug
SMC-615D-APC-100UG
About this Product
SKU:
SMC-615D-APC
Additional Names:
Microtubule associated protein tau | phospho tau
Application:
Dot Blot, Western Blot
Buffer:
95.46mM Phosphate, 2.48mM MES and 2mM EDTA
CE/IVD:
RUO
translate.label.attr.clone:
15B7
Clonality:
Monoclonal
Conjugate:
APC
Concentration:
1 mg/ml
Extra Details:
This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type.
Host:
Mouse
Immunogen:
Synthetic peptide of Wild-type Human Tau (pThr217) AA 210-224: SRTPSLP-pT-PPTREPK
Isotype:
IgG1, kappa
Purification:
Protein G Purified
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
Detects phosphorylation on Thr217
Storage Conditions:
-20[o]C
Supplier:
StressMarq Biosciences
Type:
Antibody: Monoclonal Antibody
Dot Blot analysis of Human, Mouse, Rat Tau Peptide (pThr217) showing detection of Tau protein using Mouse Anti-Tau Monoclonal Antibody, Clone 15B7 (SMC-615). Panel A: hTau 2N4R monomer (pSer214) (2 ug). Panel B: Tau peptide (pThr217) (2 ug). Panel C: Tau peptide (non phospho) (2 ug). Load: 2ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Tau Monoclonal Antibody (SMC-615) at 1:1000 for 1 hour at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:H&L at 1:4000 for 1/2 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Phospho peptide sequence: SRTPSLP-pT-PPTREPK. Non-phospho peptide sequence: SRTPSLPTPPTREPK. This sequence is the same for human, mouse, and rat.
Dot Blot analysis of Human, Mouse, Rat Tau 2N4R P301S (phospho & non-phospho) showing detection of Tau protein using Mouse Anti-Tau Monoclonal Antibody, Clone 15B7 (SMC-615). Lane 1: hTau 2N4R P301S Monomer(0.5ug). Lane 2: hTau 2N4R P301S Monomer(1.0ug). Lane 3: Recombinant hTau-441 (2N4R) P301S Monomer, Baculovirus/Sf9 Expressed (phosphorylated)(0.5ug). Lane 4: Recombinant hTau-441 (2N4R) P301S Monomer, Baculovirus/Sf9 Expressed (phosphorylated)(1.0ug). Load: 0.5ug, 1.0ug. Block: 5% BSA in TBST. Primary Antibody: Mouse Anti-Tau Monoclonal Antibody (SMC-615) at 1:4 for Overnight, 4C, with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Dot Blot performed with supernatants.