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Antibodies

ATF4 Antibody, Clone N360A/24: HRP

Product Sizes
100 ug
SMC-447D-HRP-100UG
About this Product
SKU:
SMC-447D-HRP
Additional Names:
Activating Transcription Factor 4 Antibody, ATF 4 Antibody, ATF4 protein Antibody, cAMP-dependent transcription factor ATF-4 Antibody, cAMP-responsive element-binding protein 2 Antibody, CREB 2 Antibody, CREB-2 Antibody, CREB2 Antibody, Cyclic AMP dependent transcription factor ATF 4 Antibody, Cyclic AMP response element binding protein 2 Antibody, Cyclic AMP response element binding protein 2 Antibody, Cyclic AMP-dependent transcription factor ATF-4 Antibody, Cyclic AMP-responsive element-binding protein 2 Antibody, DNA binding protein TAXREB67 Antibody, DNA binding protein TAXREB67 Antibody, DNA-binding protein TAXREB67 Antibody, Tax Responsive Enhancer Element B67 Antibody, Tax-responsive enhancer element-binding protein 67 Antibody, TAXREB67 Antibody, TXREB Antibody
Application:
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
PBS
CE/IVD:
RUO
translate.label.attr.clone:
N360A/24 (Formerly sold as S360A-24)
Clonality:
Monoclonal
Conjugate:
HRP
Concentration:
1 mg/ml
Extra Details:
Cyclic AMP-dependent transcription factor ATF-4 (ATF4) is a basic leucine-zipper (bZip) transcription factor, which regulates amino acid metabolism, DNA damage repair, chromatin remodeling, and apoptosis in response to cellular and ER stress. ATF4 works with various proteins, such as C/EBP homology protein (CHOP), aspargine synthetase (ASNS), and cAMP response element (CRE) among others to mediate cellular stress. ATF4 also regulates glucose homeostasis by suppressing beta-cell proliferation and insulin production. Furthermore, ATF4 targets the histone demethylase JMJD3 to alter chromatin structure and enhance gene transcription in response to amino acid deprivation.
Host:
Mouse
Immunogen:
Fusion protein amino acids 25-327 of human ATF4. 86% identical to rat, and 85% identical to mouse. <50% identity with ATF5.
Isotype:
IgG2a
Purification:
Protein G Purified
Reactivities:
Human, Rat
Shipping Conditions:
Blue Ice
Specificity:
Detects ~60kDa. Does not cross-react with ATF5.
Storage Conditions:
See Manual
Supplier:
StressMarq Biosciences
Type:
Antibody: Monoclonal Antibody
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-ATF4 Monoclonal Antibody, Clone N360A/24 (SMC-447). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-ATF4 Monoclonal Antibody (SMC-447) at 1:200 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) ATF4 Antibody (D) Composite.
Western Blot analysis of Rat Brain showing detection of ~39 kDa (isoform 2) ATF4 protein using Mouse Anti-ATF4 Monoclonal Antibody, Clone N360A/24 (SMC-447). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-ATF4 Monoclonal Antibody (SMC-447) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~39 kDa (isoform 2).