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Antibodies

GABA B Receptor 1 Antibody, Clone N93A/49

Product Sizes
12 ug
SMC-403-12UG
100 ug
SMC-403-100UG
About this Product
SKU:
SMC-403
Additional Names:
GABA-B receptor 1 Antibody, GABA-B-R1 Antibody, GABR1_Human Antibody, Gamma aminobutyric acid receptor 1 Antibody, GB1 Antibody, GPRC3A Antibody
Application:
Immunocytochemistry, Immunofluorescence, Western Blot
Buffer:
PBS
CE/IVD:
RUO
translate.label.attr.clone:
N93A/49 (Formerly sold as S93A-49)
Clonality:
Monoclonal
Concentration:
1 mg/ml
Extra Details:
GABA (G Gamma-aminobutyric acid) is the primary inhibitory neurotransmitter in the central nervous system and interacts with three different receptors: GABA(A), GABA(B) and GABA(C) receptor. The ionotropic GABA(A) and GABA(C) receptors are ligand-gated ion channels that produce fast inhibitory synaptic transmission. In contrast, the metabotropic GABA(B) receptor is coupled to G proteins that modulate slow inhibitory synaptic transmission (1). Functional GABA(B) receptors form heterodimers of GABA(B)R1 and GABA(B)R2 where GABA(B)R1 binds the ligand and GABA(B)R2 is the primary G protein contact site (2). Two isoforms of GABA(B)R1 have been cloned: GABA(B)R1a is a 130 kD protein and GABA(B)R1b is a 95 kD protein (3). G proteins subsequently inhibit adenyl cylase activity and modulate inositol phospholipid hydrolysis. GABA(B) receptors have both pre- and postsynaptic inhibitions: presynaptic GABA(B) receptors inhibit neurotransmitter release through suppression of high threshold calcium channels, while postsynaptic GABA(B) receptors inhibit through coupled activation of inwardly rectifying potassium channels. In addition to synaptic inhibition, GABA(B) receptors may also be involved in hippocampal long-term potentiation, slow wave sleep and muscle relaxation (1).
Host:
Mouse
Immunogen:
Fusion protein amino acids 873-977 (cytoplasmic C-terminus) of rat GABA(B)R1
Isotype:
IgG1
Purification:
Protein G Purified
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
Detects ~115kDa. No cross-reactivity against GABA(B)R2.
Storage Conditions:
-20[o]C
Supplier:
StressMarq Biosciences
Type:
Antibody: Monoclonal Antibody
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-B Receptor 1 Monoclonal Antibody, Clone N93A/49 (SMC-403). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA-B Receptor 1 Monoclonal Antibody (SMC-403) at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-B Receptor 1 Antibody (D) Composite.
Western Blot analysis of Rat brain membrane lysate showing detection of GABA B Receptor 1 protein using Mouse Anti-GABA B Receptor 1 Monoclonal Antibody, Clone N93A/49 (SMC-403). Primary Antibody: Mouse Anti-GABA B Receptor 1 Monoclonal Antibody (SMC-403) at 1:1000.