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10X TTBS pH 7.5

Product Sizes
1 l
MB-013-1L
About this Product
SKU:
MB-013
Additional Names:
TTBS, TBST, Tween Tris Buffered Saline
Application:
ELISA, Western Blot
Buffer:
See application note.
CE/IVD:
RUO
Concentration:
10X
Extra Details:
Tween Tris buffered saline is suitable for multiple applications including biological diluent buffer for antibodies or other biologics. Visit our newly expanded web site at www.rockland.com for methods using this and other buffers. This product is a 10X concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer consists of 1.0 M Tris HCl, 1.5 M Sodium Chloride and 0.1% (v/v) Tween-20 at a pH of 7.5. Meticulously prepared using ultra pure reagents dissolved in highly polished pharmaceutical grade deionized water.
Physical State:
Liquid
Shipping Conditions:
Ambient
Specificity:
10X TTBS solution was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.
Storage Conditions:
Please refer to datasheet
Supplier:
Rockland Inc
Type:
Buffers & General Consumables: Others
Western Blot showing detection of Mouse IgG, heavy and light chain, blocking in MB-013. Lane 1: MW. Lane 2: 100 ng of Mouse IgG was run on a 4-20% gel and transferred to 0.45 um nitrocellulose. Blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) 30 min at 20°C. Detection: Anti-MOUSE IgG (H&L) (GOAT) Antibody DyLight™ 488 Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins) (p/n 610-141-121) secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) and imaged using the Bio-Rad VersaDoc® 4000 MP.
Western Blot showing detection of anti-alpha tubulin blocked in MB-013. HeLa Whole Cell Lysate (10 ug) was run on a 4-20% gel, then transferred to 0.45 um nitrocellulose. After blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) for 30 min at 20°C, primary antibody was used at 1:2500 overnight at 4°C. Anti-Rabbit IgG (H&L) (GOAT) antibody IRDye800CW® (p/n 611-131-002) secondary antibody was used at 1:20,000 with Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) and imaged on the LiCor Odyssey imaging system. Arrow indicates correct 50 kDa molecular weight position expected for alpha tubulin. Lot #: 22432
10X TTBS pH 7.5 (1.0 M Tris HCl 1.5 M Sodium Chloride 0.1% (v/v) Tween-20)
AICAR suppresses ECM proteins in primary human TM cells under basal and TGF-B Beta2 stimulatory conditions. (A) Representative immunoblots of ECM proteins from CM of human TM cells treated for 24 hours with PBS vehicle or 0.5 mM AICAR and (B) integrated band intensities calculated from those immunoblots. (C) Representative immunoblots of ECM proteins from CM of human TM cells under stimulation with 2.5 ng/mL TGF-B Beta2. Cells were pre-incubated for 1 hour with PBS or 0.5 mM AICAR prior to full 24-hour treatment. (D) Mean integrated band intensities. Data in (B) and (D) are expressed as mean +/- SEM (*P < 0.05 versus PBS vehicle by Student's t-test; n = 5-7). (E) Representative 10% acrylamide gels stained with Coomassie Brilliant Blue as a loading control. Primary antibodies at 1:10,000 for SPARC, 1:1000 for TSP-1, 1:1000 for COL1 (p/n), 1:1000 for COL4 (p/n 600-401-106), 1:200 for Laminin. Washes and blocking with 1XTBST (p/n MB-013). Figure 5. PMID: 24713487.
Inhibition of the miR-29 family induces ECM synthesis. (A) Representative immunoblot and (B) densitometric analyses of ECM proteins from conditioned media of TM cells transfected with miR control, miR-29a, miR-29b, or miR-29c inhibitors. All data are expressed as the mean +/- SEM (*P < 0.05 vs. corresponding miR-Ctrl Inhibitor group; n = 5, where n refers to the number of independent experiments performed using n different primary human TM cell strains). Primary antibodies at 1:10,000 for SPARC; 1:1000 for Collagen Type I (p/n 600-401-103); 1:1000 for Collagen Type IV (p/n 600-401-106); 1:1000 for thrombospondin-1; 1:200 for laminin; and 1:2000 for fibronectin. Washes and blocking with 1XTBST (p/n MB-013). Figure 5. PMID: 21330653.