HA Epitope Tag Antibody Biotin Conjugated
Product Sizes
100 ug
600-406-384-100UG
About this Product
- SKU:
- 600-406-384
- Additional Names:
- Rabbit Anti-HA Epitope Tag Antibody Biotin Conjugated, Rabbit Anti HA Epitope Tag Antibody Biotin Conjugated
- Application:
- ELISA, Immunohistochemistry, Western Blot
- Buffer:
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
- CE/IVD:
- RUO
- Clonality:
- Polyclonal
- Conjugate:
- Biotin
- Concentration:
- 1 mg/ml
- Extra Details:
- Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. Rockland Immunochemicals produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA. Rockland Immunochemicals also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin.
- Formulation:
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- Host:
- Rabbit
- Immunogen:
- This antibody was purified from whole rabbit serum prepared by repeated immunizations with the 9-aa epitope tag peptide YPYDVPDYA (11 114-122) from hemagglutinin influenza conjugated to KLH using maleimide. A residue of cysteine was added to the carboxy terminal end to facilitate coupling.
- Isotype:
- IgG
- Physical State:
- Lyophilized
- Reactivities:
- Label/Epitope-tag
- Shipping Conditions:
- Ambient
- Specificity:
- This affinity purified antibody is directed against the HA motif and is useful in determining its presence in various assays. This polyclonal anti-HA tag antibody detects over-expressed proteins containing the HA epitope tag. To date this antibody has reacted with all HA tagged proteins so far tested. In western blotting of bacterial extracts the antibody does not cross-react with endogenous prote
- Storage Conditions:
- 2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles.
- Supplier:
- Rockland Inc
- Type:
- Antibody: Tagged Antibodies
- Manufacturer's Data Sheet:https://www.rockland.com/search/?searchString=600-406-384
