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Antibodies

BRCC45 Antibody

Product Sizes
100 ug
600-401-Z90-100UG
About this Product
SKU:
600-401-Z90
Additional Names:
BABAM2|BRCC45 Antibody, BRCC4, BRCC45, BRCA1-A complex subunit BRE, Brain and reproductive organ-expressed protein, BRISC and BRCA1-A complex member 2, BRCA1-A complex subunit BRE, BRCA1/BRCA2-containing complex subunit 451
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
BRCC45 was initially suggested to be a housekeeping protein that is highly expressed in brain and reproductive organs. Later experiments indicated BRCC45 forms a complex with the breast and ovarian predisposition proteins BRCA1 and BRCA2 as well as RAD51 and BRCC36. This complex has a ubiquitin E3 ligase activity and is thought to enhance cellular survival following DNA damage. BRCC45 has also been suggested to function as a death receptor-associated anti-apoptotic protein by inhibiting the BID-induced activation of the mitochondrial apoptotic pathway. Higher levels of BRCC45 were detected in the majority of hepatocellular carcinomas, suggesting that BRCC45 may promote tumorigenesis when overexpressed. At least three isoforms of BRCC45 are known to exist.
Formulation:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-BRCC45 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 18 amino acid synthetic peptide from near the N-terminus of human BRCC45.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human, Mouse, Rat
Shipping Conditions:
Dry Ice
Specificity:
Anti-BRCC45 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Cross reactivity with BRCC45 from other sources has not been determined.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot of BRCC45 antibody. Lane A HeLa cell lysate in the absence of blocking peptide at 1 ug/mL. Lane B: HeLa cell lysate in the presence of blocking peptide at 1 ug/mL. Load: 35 ug per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 43.5 kDa, ~85 kDa for BRCC45.
Immunohistochemistry of BRCC45 antibody. Tissue: Human brain tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: BRCC45 antibody at 2.5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: BRCC45 is nuclear and occasionally cytoplasmic. Staining: BRCC45 as precipitated red signal with hematoxylin purple nuclear counterstain.
Immunofluorescence Microscopy of BRCC45 antibody. Tissue: Human brain tissue. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: BRCC45 antibody at 20 ug/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: BRCC45 as a red fluorescent signal.