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Antibodies

BIK Antibody

Product Sizes
100 ug
600-401-Z68-100UG
About this Product
SKU:
600-401-Z68
Additional Names:
BIK|Bik Antibody, BP4, NBK, BIP1, Bcl-2-interacting killer, Apoptosis inducer NBK
Application:
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Buffer:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1 mg/ml
Extra Details:
Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by the activation of proteolytic enzymes termed caspases. Proteins that comprise the Bcl-2 family appear to control the activation of these enzymes. One such protein BIK was recently identified as an endoplasmic reticulum (ER)-residing pro-apoptotic member of the Bcl-2 homology domain-3 (BH3)-only group of the Bcl-2 family that stimulates mitochondrial release of cytochrome c following p53 induction of apoptosis. A significant fraction of BIK is found as an ER transmembrane protein, with most of the protein facing the cytosol. Restricting BIK to the ER membrane by replacing the transmembrane region with that of the ER-selective membrane anchor of cytochrome b resulted in a decreased cytochrome c release from mitochondria and a corresponding drop in cell death. Recent evidence suggests that BIK cooperates with NOXA, another BH3-only protein, to somehow enhance the activation of Bax to stimulate the rapid release of cytochrome c from mitochondria.
Formulation:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-BIK antibody was prepared from whole rabbit serum produced by repeated immunizations with a 15 amino acid synthetic peptide from near the N-terminus of human BIK.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Human, Mouse
Shipping Conditions:
Dry Ice
Specificity:
Anti-Bik Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Cross reactivity with Bik from other sources has not been determined.
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Western Blot of BIK antibody in Jurkat cell lysate. Lane A: BIK antibody at 1 ug/mL. Lane B: BIK antibody at 2 ug/mL. Load: 35 ug per lane. Primary antibody: BIK antibody at designated concentrations for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 18 kDa, 23 kDa for BIK. Other band(s): BIK splice variants and isoforms.
Immunocytochemistry of BIK antibody. Cell Type: Jurkat cells. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: BIK antibody at 1 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: BIK is located in the cell membrane and mitochondrion. Staining: BIK is stained brown with hematoxylin purple counterstain.
Immunofluorescence Microscopy of BIK antibody. Cell Type: Jurkat cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: BIK antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: BIK is located in the cell membrane and mitochondrion. Staining: BIK as green fluorescent signal.