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Antibodies

Anti-mCherry Antibody Pre-Adsorbed

Product Sizes
100 ug
600-401-P16-100UG
About this Product
SKU:
600-401-P16
Additional Names:
DsRed|rabbit anti-mCherry antibody, RFP, mCherry monomeric red fluorescent protein, Red Fluorescent Protein (RFP), rDsRed, Discosoma sp. Red Fluorescent Protein, mRFP1, mBanana, mHoneydew, mPlum, mOrange, mStrawberry, mTangerine
Application:
Detection/Quantitation, ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1.06 mg/ml
Extra Details:
mCherry Antibody is ideal for Western Blotting. Fluorescent proteins such as Discosoma Red Fluorescent Protein (and its variants), and GFP are widely used in research practice. Both commonly serve as a markers for gene expression and protein localization. DsRed was isolated from sea anemone Discosoma sp. mushroom and GFP is originated from Aequorea victoria jellyfish. As DsRed and GFP share only 19% identity, therefore, in general, anti-GFP antibodies do not recognize DsRed protein and vice versa. Structurally, Discosoma red fluorescent protein is similar to Aequorea green fluorescent protein in terms of its overall fold (a B Beta-can) and chromophore-formation chemistry. However, Discosoma red fluorescent protein undergoes an additional steps in the chromophore maturation and obligates tetrameric structure. All mCherry antibodies have been pre-absorbed to eliminate any potential cross-reactivity to human, mouse and rat serum proteins. The antibodies are also confirmed for non-reactivity to GFP protein.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
The immunogen is a mCherry mutant variant fusion protein of RFP corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Label/Epitope-tag
Shipping Conditions:
Dry Ice
Specificity:
mCherry was prepared from monospecific antiserum by immunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Expect reactivity against mCherry, RFP and its variants: tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry. Assay by immunoelectrophoresis resulted in a single precipi
Storage Conditions:
2-8[o]C Aliquot. Avoid freeze/thaw cycles. Store undiluted.
Supplier:
Rockland Inc
Type:
Antibody: Tagged Antibodies
Western Blot of Rabbit anti-mCherry antibody. Lane 1: Rockland mCherry fusion protein (reduced). Lane 2: Control dsRed reduced. Lane 3: Control mCherry reduced. Lane 4: Control BFP reduced. Lane 5: Control eGFP reduced. Lane 6: Rockland mCherry fusion protein (non-reduced). Lane 7: Control dsRed non-reduced. Lane 8: Control mCherry non-reduced. Lane 9: Control BFP non-reduced. Lane 10: Control eGFP non-reduced. Total load ~200 nanograms per lane. Load: 200ng per lane. Primary Antibody: Anti-mCherry at 1:5000 for overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:10,000 and TMBM-100. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 25.9 kDa, for mCherry and RFP, no reaction to BFP or GFP.
Western Blot of Rabbit Anti-mCherry Antibody. Lane 1: Opal Prestained Marker (p/n MB-210-0500). Lane 2: 50ng of RFP. Primary Antibody: rabbit anti-mCherry at 1ug/mL overnight at 4°C. Secondary Antibody: goat anti-Rabbit peroxidase (p/n 611-103-122) at 1:70000 for 30mins at RT. Block: BlockOut Universal Buffer (p/n MB-073). Expect band ~30 kDa.
Western Blot of Rabbit Anti-mCherry Antibody MX Hu Ms Rt. Lane 1: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 2: RFP (p/n 000-001-379)/HeLa WCL (p/n W09-000-364) [0.02ug/10ug]. Lane 3: HeLa WCL (p/n W09-000-364) [10ug]. Lane 4: RFP (p/n 000-001-379)/NIH/3T3 WCL (p/n W10-000-358) [0.02ug/10ug]. Lane 5: NIH/3T3 WCL (p/n W10-000-358) [10ug]. Lane 6: RFP (p/n 000-001-379)/PC-12 WCL (p/n W12-001-GL9) [0.02ug/10ug]. Lane 7: PC-12 WCL (p/n W12-001-GL9) [10ug]. Primary Antibody: Anti-mCherry at 1:1000 overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n 611-103-122) at 1:70,000 for 30mins at RT. Block: BlockOut Buffer (p/n MB-073). Predicted MW: ~27-30kDa.
Mapping of a minimal ML sequence required for TFIIB inhibition.A) Schematic representation of ML fragments. B) Top panel: IP of FLAG-tagged TFIIB and GFP-fused ML fragments. Bottom panel: reporter assay in HEK293 cells, where Firefly luciferase under ISRE promoter was co-transfected with EF1-A Alpha-Renilla and GFP-ML fragments. C) Top-panel: IP of FLAG-tagged TFIIB and mCherry-fused ML fragments. Bottom panel: reporter assay in HEK293 cells, where Firefly luciferase under ISRE promoter was co-transfected with EF1-A Alpha-Renilla and mCherry-ML fragments. Western blots are representative of two experiments with similar results. Bar graphs show mean and SD from three technical replicates and are representative of two experiments with similar results. Figure provided by CiteAb. Source: PLoS Pathog, PMID: 29709033.