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Antibodies

Ephrin B1, B2, B3 Antibody

Product Sizes
100 ug
600-401-MP0-100UG
About this Product
SKU:
600-401-MP0
Additional Names:
EFNB1, Efnb2, EFNB3|Rabbit Anti-Ephrin B1, B2, B3 Antibody, Ephrin-B1, Ephrin-B2, Ephrin-B3, ELK Ligand, EPLG2, LERK2, EFL3, Craniofrontonasal Syndrome (Craniofrontonasal Dysplasia), EPH-Related Receptor Transmembrane Ligand ELK-L3, Eph-Related Receptor Tyrosine Kinase Ligand 2, Eph-Related Receptor Tyrosine Kinase Ligand 8, EPH-Related Receptor Tyrosine Kinase Ligand 2, LERK-2, LERK-8, Elk-L, EFL-3, ELK-L, CFND, EFL6, EFB1, CFNS
Application:
ELISA, Immunofluorescence, Western Blot
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
CE/IVD:
RUO
Clonality:
Polyclonal
Concentration:
1.05 mg/ml
Extra Details:
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI). Ephrin B1, B2, B3 are cell surface transmembrane ligands for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binding to Eph receptors residing on adjacent cells leads to contact-dependent bidirectional signaling into neighboring. Ephrin B1 shows high affinity for the receptor tyrosine kinase EPHB1/ELK and can bind EPHB2 and EPHB3. Ephrin B3 may play a pivotal role in forebrain function. They binds to, and induces collapse of, commissural axons/growth cones in vitro. And may play a role in constraining the orientation of longitudinally projecting axons. Anti-Ephrin B1, B2, B3 Antibody is useful for researchers interested in Developmental Biology and Tyrosine Kinases/Adaptors.
Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Host:
Rabbit
Immunogen:
Anti-Ephrin B1, B2, B3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to C-terminal portion of human Ephrin B1, B2, B3 conjugated to Keyhole Limpet Hemocyanin (KLH).
Isotype:
IgG
Physical State:
Liquid
Reactivities:
Amphibian, Human, Mouse
Shipping Conditions:
Dry Ice
Specificity:
This affinity purified antibody is directed against human Ephrin B1, B2, B3. This product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with Ephrin-B1 to human, mouse, rat, and Gallus gallus; 100% homology with Ephrin-B2 to mouse; 100% homology with Ephrin-B3 to human a
Storage Conditions:
-20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
Supplier:
Rockland Inc
Type:
Antibody: Polyclonal Antibody
Immunofluorescence of Rabbit Anti-Ephrin B1-B3 Antibody. (A). Neural Plate of Xenopus laevis stage 17. (B). Knockdown neural plate of Xenopus laevis stage 17. Primary Antibody: Anti-Ephrin B1-3 at 1:5000 overnight at 2-8°C. Secondary Antibody: Anti-Rabbit IgG Fluorescent Secondary. Fixative: 4% Paraformaldehyde in PBS. Permeabilization: 0.05% Triton-X100 in PBS. Nuclear Counterstain: None. Magnification: 63X objective, Zeiss LSM710. Results: Ephrin B1 B2 B3 triple knockdown with a mixture of all three morpholinos abolished most of the signal which can be seen at expected plasma membrane and vesicle subcellular locations.
Western Blot of Rabbit Anti-Ephrin B1-B3 Antibody. Lane 1: Opal PreStain Molecular Weight Marker (p/n MB-210-0500). Lane 2: EPHRIN B1 overexpressing HEK293T Lysate (10ug) [+]. Lane 3: HEK293T Lysate (10ug) [-]. Lane 4: Mouse Kidney Lysate (p/n W10-000-T017) (35ug) [+]. Primary Antibody: Anti-Ephrin B1-B3 at 1:1000 overnight at 2-8°C. Secondary Antibody: Goat anti-Rabbit IgG MX10 HRP (p/n 611-103-122) at 1:70,000 for 30 min at RT. Block: BlockOut Buffer (p/n MB-073). Predicted MW: ~38kDa endogenous, ~37-45kDa due to glycosylation. Observed: ~46-52kDa overexpressed, ~34, ~40kDa endogenous.
Western Blot of Rabbit Anti-Ephrin B1-B3 Antibody. Lane 1: Uninjected Xenopus laevis heterogenous embryo control (20ul). Lane 2: Morpholino Knockdown of Ephrin B1/2 heterogenous embryo Lot#1 (20ul). Lane 3: Morpholino Knockdown of Ephrin B1/2 heterogenous embryo Lot#2 (20ul). Lane 4: Ephrin B1-HA overexpression heterogenous embryo lysate (20ul). Lane 5: Ephrin B2-HA overexpression heterogenous embryo lysate (20ul). Primary Antibody: Anti-Ephrin B1-B3 at 1:2000 overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n 611-103-122) at 1:5000 for 60 mins at RT. Block: 5% skim milk/TBST/0.1% Tween for 30 mins at RT. Predicted MW: ~38kDa. Observed MW: ~37-45kDa (due to glycosylation). Exposure: 10 sec. Results: Ephrin B1,2, double knockdown with a mixture of both morpholinos oligomers abolished most of the WB signal at expected size which was double checked by over-expression.
Western Blot of Rabbit Anti-Ephrin B1-B3 Antibody. Lane 1: 1st experiment: Uninjected heterogenous embryo [+]. Lane 2: 1st experiment: Ephrin B1,2 MO (2 morpholino oligomers). Lane 3: 2nd experiment: Ephrin B1,2,3 MO (3 morpholino oligomers). Lane 4: 2nd experiment: Ephrin B3 MO (1 morpholino oligomer). Lane 5: 2nd experiment: Uninjected heterogenous embryo [+]. Primary Antibody: Anti-Ephrin B1-B3 at 1:2000 overnight at 2-8°C. Secondary Antibody: Goat anti-Rabbit IgG HRP (p/n 611-103-122) 1:5000 for 60 min at RT. Block: 5% skim milk/TBST/0.1% Tween. Exposure: 10 sec. Predicted MW: ~38kDa. Observed: ~37-45kDa. Results: Ephrin B1,2,3 triple knockdown with a mixture of all three morpholinos abolished significant amount of the WB signal at expected size. The comparison of lane 3 and 4 suggests not much endogenous Ephrin B3 expression exist in this stage embryos.